Pregled bibliografske jedinice broj: 1268099
The intracellular and plasma membrane pools of PI4P control megakaryocyte maturation and proplatelet formation
The intracellular and plasma membrane pools of PI4P control megakaryocyte maturation and proplatelet formation // Research and practice in thrombosis and haemostasis, 7 (2023), 4; 100169, 10 doi:10.1016/j.rpth.2023.100169 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1268099 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The intracellular and plasma membrane pools of
PI4P control megakaryocyte maturation and
proplatelet formation
Autori
Bura, Ana ; Čabrijan, Sara ; Bertović, Ivana ; Jurak Begonja, Antonija
Izvornik
Research and practice in thrombosis and haemostasis (2475-0379) 7
(2023), 4;
100169, 10
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
megakaryocytes ; proplatelets ; PI4P ; Sac1
Sažetak
Background Megakaryocytes (MKs) develop from hematopoietic stem cells (HSCs) after stimulation by the cytokine thrombopoietin. During megakaryopoiesis, MKs enlarge, undergo the process of endomitosis and develop intracellular membranes (demarcation membrane system, DMS). During DMS formation, there is active transport of proteins, lipids, and membranes from the Golgi apparatus to the DMS. The most important phosphoinositide that controls anterograde transport from the Golgi apparatus to the plasma membrane (PM) is phosphatidylinositol- 4-monophosphate (PI4P) whose levels are controlled by the Sac1 phosphatase at the Golgi and the endoplasmic reticulum (ER). Objectives Here we investigated the role of Sac1 and PI4P in megakaryopoiesis. Results We show that in primary mouse MKs, PI4P is mostly found at the Golgi apparatus and the PM in immature MKs while in mature MKs it is in the cell periphery and at the PM. The exogenous expression of wild-type, but not C389S mutant (catalytically dead) Sac1, results in the perinuclear retention of the Golgi apparatus resembling immature MKs, with decreased ability to form proplatelets. The pharmacological inhibition of PI4P production specifically at the PM (inhibiting PI4-kinase IIIα), also resulted in a significant decrease of MKs that form proplatelets. Conclusion These results indicate that both intracellular and PM pools of PI4P mediate MK maturation and proplatelet formation.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Projekti:
HRZZ-DOK-2018-01 - Projekt razvoja karijera mladih istraživača – izobrazba novih doktora znanosti (Jurak Begonja, Antonija, HRZZ - Projekt razvoja karijera mladih istraživača – izobrazba novih doktora znanosti) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-biomed-18-188-1343 - Identifikacija novih interakcijskih partnera Vps34 u megakariopoezi (Jurak Begonja, Antonija, NadSve - UNIRI projekti 2018) ( CroRIS)
HRZZ-UIP-2014-09-2400 - Uloga fosfoinozitida u nastanku trombocita (MkPI) (Jurak Begonja, Antonija, HRZZ - 2014-09) ( CroRIS)
Ostalo-CRP/HRV15-04_EC - Deciphering the role of phosphoinositol lipids in thrombopoiesis (Jurak Begonja, Antonija, Ostalo - CRP-ICGEB Early Career Return Grant) ( CroRIS)
Ostalo-ASH-2019 - Nucleolar control of megakaryopoiesis (NucMeg) (Jurak Begonja, Antonija, Ostalo - American Society of Hematology (ASH) Global Research Award) ( CroRIS)
Ustanove:
Sveučilište u Rijeci - Odjel za biotehnologiju
Profili:
Sara Čabrijan
(autor)
Ana Bura
(autor)
Ivana Bertović
(autor)
Antonija Jurak Begonja
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Emerging Sources Citation Index (ESCI)
- Scopus