Naslov
Changes in brain myelin metabolomics of B4Galnt1 knock-out mouse caused by progressive demyelination

Autori
Mihajlović, L ; Mandić D ; Heffer, M

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
5th RECOOP International Student and 18th RECOOP Bridges in Life Sciences Conferences Book of Abstracts / Prunchunas, Edward ; Vari, Sandor G. ; Lauerova, Simona ; Vladar, Csaba - Budimpešta : RECOOP HST Association, 2023, 133-133

ISBN
978-615-6006-04-2

Skup
5th RECOOP International Student and 18th RECOOP Bridges in Life Sciences Conferences

Mjesto i datum
Budimpešta, Mađarska, 20.04.2023. - 21.04.2023

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
gangliosides ; myelin ; metabolimics ; cerebellum ; mouse

Sažetak
Using matrix-assisted laser desorption ionization time-of-flight imaging mass spectrometry (MALDI-TOF-MSI), the cerebellum of KO and WT (wild-type) 3-, 9- and 20- month-old mice (4 animals per group) was analyzed. A 9-aminoacridine matrix was applied to 16 μm thick fresh frozen tissue sections mounted on indium tin oxide coated slides. Further analysis was performed in negative and positive mode in the range 400 - 900 m/z. Data were analyzed using the software package IMAGEREVEAL (Shimadzu Inc.). Statistically significant m/z values were identified using Human Metabolome Database. Analysis was focused on cerebellar white matter as the pathophysiological substrate of the phenotype. The greatest differences were observed in negative mode between 20- month-old WT and KO mice. All statistically significant m/z values favored WT mice. Among the uniquely identified compounds were: guanosine nucleotides, cytidine triphosphate (CTP), N-acetylated amino sugars and fumarycarnitine.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA