Naslov
A model for acute poisoning detoxification – binding of aristolochic acid with various cyclodextrins

Autori
Gazdek Serdar, Nika ; Zonjić, Iva ; Nikšić-Franjić, Ivana ; Frkanec, Leo ; Piantanida, Ivo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
28HSKIKI : 28th Croatian Meeting of Chemists and Chemical Engineers and 6th Symposium Vladimir Prelog : Book of Abstracts / Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023, 73-73

Skup
28th Croatian Meeting of Chemists and Chemical Engineers (28HSKIKI)

Mjesto i datum
Rovinj, Hrvatska, 28.03.2023. - 31.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aristolochic acid toxin ; cyclodextrins ; detoxification

Sažetak
Aristolochic acid I (ARI) is a planar extended aromatic molecule, well-known for its carcinogenic, mutagenic and nephrotoxic activity and most commonly linked to the Europe Balkan region (particularly the Danube – Black Sea basin), causing severe endemic nephropathy. Low aqueous solubility of ARI leads commonly to consequent hydrophobicdriven binding to serum albumin in the blood, thus being distributed over the organism, strongly supported by ARI hydrophobicity and consequent slow clearance kinetics. The application of macrocyclic compounds as host molecules in biomedicine is generally wellknown and intensively studied. We showed that aristolochic acid toxin (ARI) and its fluorescent analogue form inclusion complexes with various cyclodextrins (CDs), including the clinically used γ-CD: Sugammadex. The binding affinity of ARI towards CDs was determined with UV-Vis and fluorescence spectroscopy in a phosphate buffer solution. Affinity varied on CD size and rim modifications, whereby the stability of the ARI/Sugammadex complex was 2-fold greater than ARI binding to serum albumin (BSA). These results are a proof-ofconcept that CDs could be used as a first aid upon acute intoxication with ARI by binding at least part of the toxin in competition with serum albumin and significantly reducing ARI absorption in the organism.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA