Tff3−/− Knock-Out Mice with Altered Lipid Metabolism Exhibit a Lower Level of Inflammation following the Dietary Intake of Sodium Chloride for One Week

Kolobarić, Nikolina; Mihalj, Martina; Kozina, Nataša; Matić, Anita; Mihaljević, Zrinka; Drenjančević, Ines

doi:10.3390/ijms24087315

Pregled bibliografske jedinice broj: 1266875

Tff3−/− Knock-Out Mice with Altered Lipid Metabolism Exhibit a Lower Level of Inflammation following the Dietary Intake of Sodium Chloride for One Week

Kolobarić, Nikolina; Mihalj, Martina; Kozina, Nataša; Matić, Anita; Mihaljević, Zrinka; Drenjančević, Ines

Tff3−/− Knock-Out Mice with Altered Lipid Metabolism Exhibit a Lower Level of Inflammation following the Dietary Intake of Sodium Chloride for One Week // International journal of molecular sciences, 24 (2023), 8; 7315, 20 doi:10.3390/ijms24087315 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1266875 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Tff3−/− Knock-Out Mice with Altered Lipid Metabolism Exhibit a Lower Level of Inflammation following the Dietary Intake of Sodium Chloride for One Week

Autori
Kolobarić, Nikolina ; Mihalj, Martina ; Kozina, Nataša ; Matić, Anita ; Mihaljević, Zrinka ; Drenjančević, Ines

Izvornik
International journal of molecular sciences (1661-6596) 24 (2023), 8; 7315, 20

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
inflammation ; integrins ; knock-out ; sodium chloride ; trefoil factor-3

Sažetak
A high salt intake causes hemodynamic changes and promotes immune response through cell activation and cytokine production, leading to pro- inflammatory conditions. Transgenic Tff3−/− knock- out mice (TFF3ko) (n = 20) and wild-type mice (WT) (n = 20) were each divided into the (1) low-salt (LS) group and (2) high-salt (HS) group. Ten-week- old animals were fed with standard rodent chow (0.4% NaCl) (LS) or food containing 4% NaCl (HS) for one week (7 days). Inflammatory parameters from the sera were measured by Luminex assay. The integrin expression and rates of T cell subsets of interest from the peripheral blood leukocytes (PBLs) and mesenteric lymph nodes (MLNs) were measured using flow cytometry. There was a significant increase in high-sensitivity C reactive protein (hsCRP) only in the WT mice following the HS diet, while there were no significant changes in the serum levels of IFN-γ, TNF-α, IL-2, IL-4, or IL-6 as a response to treatment in either study groups. The rates of CD4+CD25+ T cells from MLNs decreased, while CD3+γδTCR+ from peripheral blood increased following the HS diet only in TFF3ko. γδTCR expressing T cell rates decreased in WT following the HS diet. The CD49d/VLA-4 expression decreased in the peripheral blood leukocytes in both groups following the HS diet. CD11a/LFA-1 expression significantly increased only in the peripheral blood Ly6C−CD11ahigh monocytes in WT mice following salt loading. In conclusion, salt- loading in knock-out mice caused a lower level of inflammatory response compared with their control WT mice due to gene depletion.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA

Projekti:
HRZZ-IP-2014-09-6380 - Poremećena vazorelaksacija i endotelno-leukocitna interakcija (ELI) u razvoju aterosklerotskih lezija (V-ELI Athero) (Drenjančević, Ines, HRZZ - 2014-09) ( CroRIS)

Profili:

Anita Matić (autor)