Pregled bibliografske jedinice broj: 1266863
The miR-20a/miR-92b Profile Is Associated with Circulating γδ T-Cell Perturbations in Mild Psoriasis
The miR-20a/miR-92b Profile Is Associated with Circulating γδ T-Cell Perturbations in Mild Psoriasis // International journal of molecular sciences, 24(5) (2023), 4323; 1-15 doi:10.3390/ijms24054323 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1266863 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The miR-20a/miR-92b Profile Is Associated with
Circulating γδ T-Cell Perturbations in Mild
Psoriasis
Autori
Tokić, Stana ; Jirouš, Maja ; Plužarić, Vera ; Mihalj, Martina ; Šola, Marija ; Tolušić Levak, Maja ; Glavaš, Kristina ; Balogh, Peter ; Štefanić, Mario
Izvornik
International journal of molecular sciences (1422-0067) 24(5)
(2023), 4323;
1-15
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
hsa-mir-20a ; hsa-mir-92b ; hsa-mir-29a ; hsa-let-7c ; psoriasis vulgaris ; γδ T cells
Sažetak
Psoriasis vulgaris (PV) is an autoinflammatory dermatosis of unknown etiology. Current evidence suggests a pathogenic role of γδT cells, but the growing complexity of this population has made the offending subset difficult to pinpoint. The work on γδTCRint and γδTCRhi subsets, which express intermediate and high levels of γδTCR at their surface, respectively, is particularly scarce, leaving their inner workings in PV essentially unresolved. We have shown here that the γδTCRint/ γδTCRhi cell composition and their transcriptome are related to the differential miRNA expression by performing a targeted miRNA and mRNA quantification (RT-qPCR) in multiplexed, flow- sorted γδ blood T cells from healthy controls (n = 14) and patients with PV (n = 13). A significant loss of miR-20a in bulk γδT cells (~fourfold decrease, PV vs. controls) largely mirrored increasing Vδ1-Vδ2- and γδintVδ1-Vδ2- cell densities in the bloodstream, culminating in a relative excess of γδintVδ1-Vδ2- cells for PV. Transcripts encoding DNA-binding factors (ZBTB16), cytokine receptors (IL18R1), and cell adhesion molecules (SELPLG) were depleted in the process, closely tracking miR20a availability in bulk γδ T- cell RNA. Compared to controls, PV was also associated with enhanced miR-92b expression (~13- fold) in bulk γδT cells that lacked association with the γδT cell composition. The miR-29a and let-7c expressions remained unaltered in case– control comparisons. Overall, our data expand the current landscape of the peripheral γδT cell composition, underlining changes in its mRNA/miRNA transcriptional circuits that may inform PV pathogenesis.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
HRZZ-UIP-2019-04-3494 - NGS analiza transkriptoma MAIT i yd T limfocita: fenotip, funkcija i raznolikost TCR klonova u pozadini razvoja vulgarne psorijaze (NGSmyPHENOTIP) (Tokić, Stana, HRZZ - HRZZ UIP-2019-04) ( CroRIS)
MZO Ustanova-Medicinski fakultet, Osijek-MEFOS-IP15-2020 - NGS analiza raznolikosti TCRβ repertoara perifernih TCRVα7.2+MR1+ MAIT stanica u oboljelih od vulgarne psorijaze (Tokić, Stana, MZO Ustanova - MEFOS-IP-2020) ( CroRIS)
MZO Ustanova-Medicinski fakultet, Osijek-MEFOS-IP6-2021 - RNASeq analiza imunološkog transkriptoma γδT stanica u vulgarnoj psorijazi (Tokić, Stana, MZO Ustanova - MEFOS-IP-2021) ( CroRIS)
Ustanove:
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek
Profili:
Stana Tokić
(autor)
Kristina Glavas
(autor)
Martina Mihalj
(autor)
Maja Jirouš
(autor)
Mario Štefanić
(autor)
Maja Tolušić Levak
(autor)
Marija Šola
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE