Naslov
Evaluation of the Interactions between Mumps Virus and Guinea Pig

Autori
Lang Balija, Maja ; Štimac, Adela ; Košutić Gulija, Tanja ; Gudan Kurilj, Andrea ; Bekavac, Ana ; Plećaš, Ante ; Halassy, Beata ; Jagušić, Maja ; Forčić, Dubravko

Izvornik
Journal of Virology (0022-538X) 5 (2023); 0035923, 17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
guinea pig ; host-pathogen interaction ; mumps virus ; primary cell culture ; sialic acid

Sažetak
Mumps is a highly contagious viral disease that can be prevented by vaccination. In the last decade, we have encountered repeated outbreaks of mumps in highly vaccinated populations, which call into question the effectiveness of available vaccines. Animal models are crucial for understanding virus-host interactions, and viruses such as mumps virus (MuV), whose only natural host is the human, pose a particular challenge. In our study, we examined the interaction between MuV and the guinea pig. Our results present the first evidence that guinea pigs of the Hartley strain can be infected in vivo after intranasal and intratesticular inoculation. We observed a significant viral replication in infected tissues up to 5 days following infection and induction of cellular and humoral immune responses as well as histopathological changes in infected lungs and testicles, without clinical signs of disease. Transmission of the infection through direct contact between animals was not possible. Our results demonstrate that guinea pigs and guinea pig primary cell cultures represent a promising model for immunological and pathogenetic studies of the complex MuV infection.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA