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Pregled bibliografske jedinice broj: 1266019

Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains


Mardešić, Ivan; Boban, Zvonimir; Subczynski, Witold Karol; Raguž, Marija
Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains // Membranes, 13 (2023), 3; 320, 21 doi:10.3390/membranes13030320 (međunarodna recenzija, članak, znanstveni)


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Naslov
Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains

Autori
Mardešić, Ivan ; Boban, Zvonimir ; Subczynski, Witold Karol ; Raguž, Marija

Izvornik
Membranes (2077-0375) 13 (2023), 3; 320, 21

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
plasma membrane ; cholesterol ; phospholipids ; liposomes ; supported lipid bilayer ; cholesterol bilayer domains ; cholesterol crystals ; saturation recovery electron paramagnetic resonance ; atomic force microscopy ; X-ray diffraction

Sažetak
Cholesterol (Chol) is an essential component of animal cell membranes and is most abundant in plasma membranes (PMs) where its concentration typically ranges from 10 to 30 mol%. However, in red blood cells and Schwann cells, PMs Chol content is as high as 50 mol%, and in the PMs of the eye lens fiber cells, it can reach up to 66 mol%. Being amphiphilic, Chol molecules are easily incorporated into the lipid bilayer where they affect the membrane lateral organization and transmembrane physical properties. In the aqueous phase, Chol cannot form free bilayers by itself. However, pure Chol bilayer domains (CBDs) can form in lipid bilayer membranes with the Chol content exceeding 50 mol%. The range of Chol concentrations surpassing 50 mol% is less frequent in biological membranes and is consequently less investigated. Nevertheless, it is significant for the normal functioning of the eye lens and understanding how Chol plaques form in atherosclerosis. The most commonly used membrane models are unilamellar and multilamellar vesicles (MLVs) and supported lipid bilayers (SLBs). CBDs have been observed directly using confocal microscopy, X-ray reflectometry and saturation recovery electron paramagnetic resonance (SR EPR). Indirect evidence of CBDs has also been reported by using atomic force microscopy (AFM) and fluorescence recovery after photobleaching (FRAP) experiments. The overall goal of this review is to demonstrate the advantages and limitations of the various membrane models and experimental techniques suitable for the detection and investigation of the lateral organization, function and physical properties of CBDs.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekti:
IP-2019-04-1958 - Otkrivanje organizacije i funkcije kolesterolove domene u plasma membrani fibroznih stanica leće oka uz primjenu fluorescentnih metoda (BioPhyCBD) (Raguž, Marija, HRZZ - 2019-04) ( CroRIS)

Ustanove:
Prirodoslovno-matematički fakultet, Split,
Medicinski fakultet, Split

Profili:

Avatar Url Marija Raguž (autor)

Avatar Url Zvonimir Boban (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.mdpi.com

Citiraj ovu publikaciju:

Mardešić, Ivan; Boban, Zvonimir; Subczynski, Witold Karol; Raguž, Marija
Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains // Membranes, 13 (2023), 3; 320, 21 doi:10.3390/membranes13030320 (međunarodna recenzija, članak, znanstveni)
Mardešić, I., Boban, Z., Subczynski, W. & Raguž, M. (2023) Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains. Membranes, 13 (3), 320, 21 doi:10.3390/membranes13030320.
@article{article, author = {Marde\v{s}i\'{c}, Ivan and Boban, Zvonimir and Subczynski, Witold Karol and Ragu\v{z}, Marija}, year = {2023}, pages = {21}, DOI = {10.3390/membranes13030320}, chapter = {320}, keywords = {plasma membrane, cholesterol, phospholipids, liposomes, supported lipid bilayer, cholesterol bilayer domains, cholesterol crystals, saturation recovery electron paramagnetic resonance, atomic force microscopy, X-ray diffraction}, journal = {Membranes}, doi = {10.3390/membranes13030320}, volume = {13}, number = {3}, issn = {2077-0375}, title = {Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains}, keyword = {plasma membrane, cholesterol, phospholipids, liposomes, supported lipid bilayer, cholesterol bilayer domains, cholesterol crystals, saturation recovery electron paramagnetic resonance, atomic force microscopy, X-ray diffraction}, chapternumber = {320} }
@article{article, author = {Marde\v{s}i\'{c}, Ivan and Boban, Zvonimir and Subczynski, Witold Karol and Ragu\v{z}, Marija}, year = {2023}, pages = {21}, DOI = {10.3390/membranes13030320}, chapter = {320}, keywords = {plasma membrane, cholesterol, phospholipids, liposomes, supported lipid bilayer, cholesterol bilayer domains, cholesterol crystals, saturation recovery electron paramagnetic resonance, atomic force microscopy, X-ray diffraction}, journal = {Membranes}, doi = {10.3390/membranes13030320}, volume = {13}, number = {3}, issn = {2077-0375}, title = {Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains}, keyword = {plasma membrane, cholesterol, phospholipids, liposomes, supported lipid bilayer, cholesterol bilayer domains, cholesterol crystals, saturation recovery electron paramagnetic resonance, atomic force microscopy, X-ray diffraction}, chapternumber = {320} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati:





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