Naslov
Development of a planar pritned lactate biosensor

Autori
Ivanišević, Irena ; Kassal, Petar

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
28th Croatian Meeting of Chemists and Chemical Engineers and 6th Symposium Vladimir Prelog: Book of Abstracts / Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023, 79-79

Skup
28th Croatian Meeting of Chemists and Chemical Engineers and 6th Symposium Vladimir Prelog

Mjesto i datum
Rovinj, Hrvatska, 28.03.2023. - 31.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
lactate ; lactate oxidase ; biosensor ; inkjet printing

Sažetak
In recent years, the development of personalized and wearable sensing platforms for non-invasive in situ monitoring of lactate in sweat has gained importance in sports and medical diagnostics [1]. Amperometric enzymatic sensors are particularly convenient for lactate quantification ; because they are relatively inexpensive and have high sensitivity, they are ideal for commercial scale production. Under physiological conditions, lactate can be oxidized by enzymes to pyruvate, producing hydrogen peroxide (H2O2) as a byproduct. The sensing mechanism underlying lactate detection follows the physiological pathway and, for first- generation biosensors, is based on the usage of enzyme lactate oxidase (LOx), often coupled with a H2O2 electrocatalyst, such as Prussian blue (PB), which mediates current output at mild applied potentials [2]. This work presents an enzyme sensor fabricated over a glassy carbon electrode (GCE) for measuring lactate in synthetic sweat. To establish a lactate sensing platform, multilayer films were formed by the simple layer-by-layer (LbL) method in the following sequence: (i) PB as a redox mediator ; (ii) LOx as an enzyme, and (iii) chitosan as an immobilization matrix and protective outer layer serving as lactate diffusion medium. Since each layer effects the performance of biosensor, each fabrication step is carefully and systematically examined. First, the thickness of the PB layer was optimized by adjusting the concentrations of the precursor solutions to yield maximum current output and high catalytic activity towards H2O2. Second, the dynamic amperometric response and the corresponding calibration curves at increasing lactate concentrations in 0.01 M phosphate buffer using the chitosan-LOx-PB/GCE biosensor were provided. The influence of pH and ionic strength were also investigated. The advantages of the presented sensor are its ease of fabrication, including in situ chemical synthesis of the PB mediator (avoiding the time- and energy-consuming electrochemical deposition), and the use of the biocompatible chitosan as an enzyme protective layer. Finally, the biosensor design was transferred to printed electrodes, yielding miniaturized planar flexible sensors.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA