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Pregled bibliografske jedinice broj: 1265907

Preparation of molecularly imprinted polymers for the extraction of fulvestrant from human plasma samples


Mutavdžić Pavlović, Dragana; Turković, Lu; Mlinarić, Zvonimir; Javorić, Kristina; Vuić, Juraj; Skenderović, Anamarija; Makarić, Sandro; Radić, Vladimir; Silovski, Tajana; Sertić, Miranda
Preparation of molecularly imprinted polymers for the extraction of fulvestrant from human plasma samples // 28th Croatian Meeting of Chemists and Chemical Engineers Book of Abstracts / Rogošić, Marko (ur.).
Zagreb: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023. str. 108-108 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1265907 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Preparation of molecularly imprinted polymers for the extraction of fulvestrant from human plasma samples

Autori
Mutavdžić Pavlović, Dragana ; Turković, Lu ; Mlinarić, Zvonimir ; Javorić, Kristina ; Vuić, Juraj ; Skenderović, Anamarija ; Makarić, Sandro ; Radić, Vladimir ; Silovski, Tajana ; Sertić, Miranda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
28th Croatian Meeting of Chemists and Chemical Engineers Book of Abstracts / Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023, 108-108

Skup
28th Croatian Meeting of Chemists and Chemical Engineers

Mjesto i datum
Rovinj, Hrvatska, 28.03.2023. - 31.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
plasma sample ; molecularly imprinted polymers ; breast cancer

Sažetak
In the development of bioanalytical methods, selective sample preparation is of great importance. It is necessary to extract the analytes of interest, which are often present in very low concentrations, from a complex matrix that can both affect the obtained results and damage the analytical instrument. Molecularly imprinted polymers (MIP) show great potential for increasing selectivity in the extraction of different molecules from such complex samples, as they are synthesized with the analyte of interest as a template. Hormone-positive, human epidermal growth factor receptor-2-negative breast cancer is treated with therapeutic combinations of palbociclib, ribociclib, or abemaciclib with anastrozole, letrozole or fulvestrant. Due to the observed inter-patient variability in plasma concentrations, numerous new bioanalytical methods are currently being developed to determine these drugs in patient samples. [1, 2] Initially, various solid phase extraction (SPE) sorbents were tested for extraction from plasma. It was found that fulvestrant had the lowest extraction recoveries gains with all sorbents tested (<75%). Therefore, different MIPs were synthesized to improve the extraction using 2-hydroxyethyl methacrylate (HEMA), 4- vinylpyridine (VPY) and methacrylic acid (MAA) as different functional monomers. Ethylene glycol dimethacrylate was used for crosslinking, and 2, 2’-azobisisobutyronitrile was used as the polymerization initiator. The samples were analyzed using Agilent 1290 UHPLC couple to 6470 triple quadrupole MS detector. A Waters XBridge phenyl column (150 × 4.6 mm, 2.5 μm) was used with methanol, water and formic acid in gradient elution. The obtained extraction recoveries of fulvestrant were greatly increased (75-108%) with the tested MIP sorbents, especially when eluted successively with 2% formic acid in methanol and methanol. Finally, the MIP sorbent synthesized with HEMA monomers was coupled with an HLB sorbent to achieve simultaneous extraction of all six analytes. In this combination, using the same eluents, the recovery of fulvestrant was significantly improved (≈99%) compared with HLB alone (75%). Acknowledgement This work has been fully supported by the Croatian Science Foundation through projects UIP2019-04-8461 and DOK-2021-02- 4595, and the European Regional Development Fund, project Farminova, KK.01.1.1.02.0021.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo, Farmacija



POVEZANOST RADA


Projekti:
UIP-2019-04-8461 - Nova bioanalitička rješenja za personalizaciju terapije raka dojke (OncoBioAnalytics) (Sertić, Miranda, HRZZ - 2019-04) ( CroRIS)
EK-EFRR-KK.01.1.1.02.0021 - Jačanje znanstveno-istraživačkih i inovacijskih kapaciteta Farmaceutsko-biokemijskog fakulteta Sveučilišta u Zagrebu (FarmInova) (Lovrić, Jasmina, EK - Ulaganje u organizacijsku reformu i infrastrukturu u sektoru istraživanja, razvoja i inovacija) ( CroRIS)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Pliva-Istraživački institut,
Fakultet kemijskog inženjerstva i tehnologije, Zagreb,
Klinički bolnički centar Zagreb

Poveznice na cjeloviti tekst rada:

www.hdki.hr

Citiraj ovu publikaciju:

Mutavdžić Pavlović, Dragana; Turković, Lu; Mlinarić, Zvonimir; Javorić, Kristina; Vuić, Juraj; Skenderović, Anamarija; Makarić, Sandro; Radić, Vladimir; Silovski, Tajana; Sertić, Miranda
Preparation of molecularly imprinted polymers for the extraction of fulvestrant from human plasma samples // 28th Croatian Meeting of Chemists and Chemical Engineers Book of Abstracts / Rogošić, Marko (ur.).
Zagreb: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023. str. 108-108 (poster, međunarodna recenzija, sažetak, znanstveni)
Mutavdžić Pavlović, D., Turković, L., Mlinarić, Z., Javorić, K., Vuić, J., Skenderović, A., Makarić, S., Radić, V., Silovski, T. & Sertić, M. (2023) Preparation of molecularly imprinted polymers for the extraction of fulvestrant from human plasma samples. U: Rogošić, M. (ur.)28th Croatian Meeting of Chemists and Chemical Engineers Book of Abstracts.
@article{article, author = {Mutavd\v{z}i\'{c} Pavlovi\'{c}, Dragana and Turkovi\'{c}, Lu and Mlinari\'{c}, Zvonimir and Javori\'{c}, Kristina and Vui\'{c}, Juraj and Skenderovi\'{c}, Anamarija and Makari\'{c}, Sandro and Radi\'{c}, Vladimir and Silovski, Tajana and Serti\'{c}, Miranda}, editor = {Rogo\v{s}i\'{c}, M.}, year = {2023}, pages = {108-108}, keywords = {plasma sample, molecularly imprinted polymers, breast cancer}, title = {Preparation of molecularly imprinted polymers for the extraction of fulvestrant from human plasma samples}, keyword = {plasma sample, molecularly imprinted polymers, breast cancer}, publisher = {Hrvatsko dru\v{s}tvo kemijskih in\v{z}enjera i tehnologa (HDKI)}, publisherplace = {Rovinj, Hrvatska} }
@article{article, author = {Mutavd\v{z}i\'{c} Pavlovi\'{c}, Dragana and Turkovi\'{c}, Lu and Mlinari\'{c}, Zvonimir and Javori\'{c}, Kristina and Vui\'{c}, Juraj and Skenderovi\'{c}, Anamarija and Makari\'{c}, Sandro and Radi\'{c}, Vladimir and Silovski, Tajana and Serti\'{c}, Miranda}, editor = {Rogo\v{s}i\'{c}, M.}, year = {2023}, pages = {108-108}, keywords = {plasma sample, molecularly imprinted polymers, breast cancer}, title = {Preparation of molecularly imprinted polymers for the extraction of fulvestrant from human plasma samples}, keyword = {plasma sample, molecularly imprinted polymers, breast cancer}, publisher = {Hrvatsko dru\v{s}tvo kemijskih in\v{z}enjera i tehnologa (HDKI)}, publisherplace = {Rovinj, Hrvatska} }




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