Naslov
Oxidative stress markers measured in the kidney of male Wistar rats treated with sterigmatocystin

Autori
Šunić, Iva ; Rašić, Dubravka ; Peraica, Maja ; Jakšić, Daniela ; Šegvić Klarić, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Arhiv za higijenu rada i Toksikologiju / - Zagreb, 2022, A41-A41

Skup
Power of Fungi and Mycotoxins in the Midst of Climate Change, International Symposium

Mjesto i datum
Koprivnica, Hrvatska, 16.09.2022. - 17.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antioxidative enzymes ; in vivo study ; malondialdehyde ; mycotoxins ; nephrotoxicity

Sažetak
Sterigmatocystin is a mycotoxin structurally similar to aflatoxin B1 and a precursor to its biosynthesis. It is synthesized by 55 different species of mould that belong to phylogenetically and ecologically diverse genera. Sterygmatocistin can be found in various foodstuffs, even after processing, as well as in indoor humid spaces and dust. The LD50 for oral exposure is 160 mg/kg b.w. The mechanism of action is associated with the formation of exo-epoxides and the formation of DNA adducts. Therefore, the assumed mechanism of action is related to the occurrence of oxidative stress, which can have a great impact on health. This study investigates the role of sterigmatocystin in the generation of oxidative stress in the kidneys of male Wistar rats. Rats were treated once with steigmatocystin at three different doses: 1/4, 1/8, and 1/16 LD50 (40, 20, and 10 mg/kg b.w.). Parameters of oxidative stress that have been measured are activities of the enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), the concentration of reduced glutathione (GSH) and malondialdehyde (MDA). Controls included solvent control corn oil and water. When compared to the controls, a statistically significant difference was observed in MDA concentration and SOD activity. The difference was observed for all three concentrations in MDA and 1/16 and 1/4 treatments in SOD. A significant difference in the activity of CAT and GPx and concentration of GSH was not noted in kidneys, although CAT showed an increasing and GPx decreasing trend. The results showed that there was a high occurrence of oxidative processes (SOD) and tissue damage (MDA) in the kidneys and that they were sensitive to the occurrence of oxidative stress caused by sterigmatocystin. However, further studies are needed to discover the mechanism of sterigmatocystin toxicity.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

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