Pregled bibliografske jedinice broj: 1264817
Selection of the natural deep eutectic solvent for extraction of the Aspergillus oryzae lipase from liquid formulations
Selection of the natural deep eutectic solvent for extraction of the Aspergillus oryzae lipase from liquid formulations // Book of Abstracts 28th Croatian Meeting of Chemists and Chemical Engineers / Rogošić, Marko (ur.).
Zagreb: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023. str. 34-34 (predavanje, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 1264817 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Selection of the natural deep eutectic solvent for
extraction of the Aspergillus
oryzae lipase from liquid formulations
Autori
Bekavac, Nikša ; Jurinjak Tušek, Ana ; Radović, Mia ; Cvjetko Bubalo, Marina ; Zelić, Bruno ; Šalić, Anita
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts 28th Croatian Meeting of Chemists and Chemical Engineers
/ Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023, 34-34
Skup
28th Croatian Meeting of Chemists and Chemical Engineers
Mjesto i datum
Rovinj, Hrvatska, 28.03.2023. - 31.03.2023
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Domaća recenzija
Ključne riječi
natural deep eutectic solvent ; lipase ; extraction
Sažetak
The development of clean, sustainable, and effective extraction techniques has received much attention in recent years due to the growing interest in green extraction. For downstream processing (extraction) of biomolecules, natural deep eutectic solvents (NADES) are considered as efficient, adaptable, and significant new green solvents. Their advantages include low-cost constitutive elements such as choline chloride, which serves as a representative hydrogen bond acceptor (HBA), and naturally occurring hydrogen bond donors (HBDs), including different sugars, alcohols, and amides. Because of their stability, biodegradability, non-toxicity, and low volatility, NADES are of particular interest and are considered as "green solvents" within the context of green chemistry. The aqueous two-phase system (ATPS) based on NADES was investigated as a possible extraction medium for the purification of Aspergillus oryzae lipase from raw liquid enzyme formulations. Choline-chloride was selected as the hydrogen bond acceptor while fructose, glucose, glycerol, malonic acid, urea, thiourea and ethylene glycole were considered as hydrogen bond donors. After the NADESs were prepared and characterized (pH, density, and viscosity) the σ- profile for each NADES was determined. The extraction process was carried out in a batch extractor and the extraction efficiency and enzyme activity in each NADES based aqueous two phase system (ATPS) were determined. Based on the experimental results and σ-profiles, a model for the prediction of enzyme activity and extraction efficiency in different ATPSs was developed using the COSMO-RS program. The obtained model was validated on a set of independent experiments performed with ATPS based on different NADES.
Izvorni jezik
Engleski
Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija
POVEZANOST RADA
Projekti:
UNESCO--4500461754 - Kontinuirana ekstrakcija proteina u mikroekstraktoru vodenim dvofaznim sustavima na bazi prirodnih eutektičkih otapala: od otpada do vrijednog proizvoda (Šalić, Anita, UNESCO ) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb,
Fakultet kemijskog inženjerstva i tehnologije, Zagreb,
Sveučilište Sjever, Koprivnica
Profili:
Marina Cvjetko Bubalo
(autor)
Mia Radović
(autor)
Ana Jurinjak Tušek
(autor)
Anita Šalić
(autor)
Bruno Zelić
(autor)