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Pregled bibliografske jedinice broj: 1264817

Selection of the natural deep eutectic solvent for extraction of the Aspergillus oryzae lipase from liquid formulations


Bekavac, Nikša; Jurinjak Tušek, Ana; Radović, Mia; Cvjetko Bubalo, Marina; Zelić, Bruno; Šalić, Anita
Selection of the natural deep eutectic solvent for extraction of the Aspergillus oryzae lipase from liquid formulations // Book of Abstracts 28th Croatian Meeting of Chemists and Chemical Engineers / Rogošić, Marko (ur.).
Zagreb: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023. str. 34-34 (predavanje, domaća recenzija, sažetak, znanstveni)


CROSBI ID: 1264817 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Selection of the natural deep eutectic solvent for extraction of the Aspergillus oryzae lipase from liquid formulations

Autori
Bekavac, Nikša ; Jurinjak Tušek, Ana ; Radović, Mia ; Cvjetko Bubalo, Marina ; Zelić, Bruno ; Šalić, Anita

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts 28th Croatian Meeting of Chemists and Chemical Engineers / Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023, 34-34

Skup
28th Croatian Meeting of Chemists and Chemical Engineers

Mjesto i datum
Rovinj, Hrvatska, 28.03.2023. - 31.03.2023

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
natural deep eutectic solvent ; lipase ; extraction

Sažetak
The development of clean, sustainable, and effective extraction techniques has received much attention in recent years due to the growing interest in green extraction. For downstream processing (extraction) of biomolecules, natural deep eutectic solvents (NADES) are considered as efficient, adaptable, and significant new green solvents. Their advantages include low-cost constitutive elements such as choline chloride, which serves as a representative hydrogen bond acceptor (HBA), and naturally occurring hydrogen bond donors (HBDs), including different sugars, alcohols, and amides. Because of their stability, biodegradability, non-toxicity, and low volatility, NADES are of particular interest and are considered as "green solvents" within the context of green chemistry. The aqueous two-phase system (ATPS) based on NADES was investigated as a possible extraction medium for the purification of Aspergillus oryzae lipase from raw liquid enzyme formulations. Choline-chloride was selected as the hydrogen bond acceptor while fructose, glucose, glycerol, malonic acid, urea, thiourea and ethylene glycole were considered as hydrogen bond donors. After the NADESs were prepared and characterized (pH, density, and viscosity) the σ- profile for each NADES was determined. The extraction process was carried out in a batch extractor and the extraction efficiency and enzyme activity in each NADES based aqueous two phase system (ATPS) were determined. Based on the experimental results and σ-profiles, a model for the prediction of enzyme activity and extraction efficiency in different ATPSs was developed using the COSMO-RS program. The obtained model was validated on a set of independent experiments performed with ATPS based on different NADES.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija



POVEZANOST RADA


Projekti:
UNESCO--4500461754 - Kontinuirana ekstrakcija proteina u mikroekstraktoru vodenim dvofaznim sustavima na bazi prirodnih eutektičkih otapala: od otpada do vrijednog proizvoda (Šalić, Anita, UNESCO ) ( CroRIS)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb,
Fakultet kemijskog inženjerstva i tehnologije, Zagreb,
Sveučilište Sjever, Koprivnica


Citiraj ovu publikaciju:

Bekavac, Nikša; Jurinjak Tušek, Ana; Radović, Mia; Cvjetko Bubalo, Marina; Zelić, Bruno; Šalić, Anita
Selection of the natural deep eutectic solvent for extraction of the Aspergillus oryzae lipase from liquid formulations // Book of Abstracts 28th Croatian Meeting of Chemists and Chemical Engineers / Rogošić, Marko (ur.).
Zagreb: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023. str. 34-34 (predavanje, domaća recenzija, sažetak, znanstveni)
Bekavac, N., Jurinjak Tušek, A., Radović, M., Cvjetko Bubalo, M., Zelić, B. & Šalić, A. (2023) Selection of the natural deep eutectic solvent for extraction of the Aspergillus oryzae lipase from liquid formulations. U: Rogošić, M. (ur.)Book of Abstracts 28th Croatian Meeting of Chemists and Chemical Engineers.
@article{article, author = {Bekavac, Nik\v{s}a and Jurinjak Tu\v{s}ek, Ana and Radovi\'{c}, Mia and Cvjetko Bubalo, Marina and Zeli\'{c}, Bruno and \v{S}ali\'{c}, Anita}, editor = {Rogo\v{s}i\'{c}, M.}, year = {2023}, pages = {34-34}, keywords = {natural deep eutectic solvent, lipase, extraction}, title = {Selection of the natural deep eutectic solvent for extraction of the Aspergillus oryzae lipase from liquid formulations}, keyword = {natural deep eutectic solvent, lipase, extraction}, publisher = {Hrvatsko dru\v{s}tvo kemijskih in\v{z}enjera i tehnologa (HDKI)}, publisherplace = {Rovinj, Hrvatska} }
@article{article, author = {Bekavac, Nik\v{s}a and Jurinjak Tu\v{s}ek, Ana and Radovi\'{c}, Mia and Cvjetko Bubalo, Marina and Zeli\'{c}, Bruno and \v{S}ali\'{c}, Anita}, editor = {Rogo\v{s}i\'{c}, M.}, year = {2023}, pages = {34-34}, keywords = {natural deep eutectic solvent, lipase, extraction}, title = {Selection of the natural deep eutectic solvent for extraction of the Aspergillus oryzae lipase from liquid formulations}, keyword = {natural deep eutectic solvent, lipase, extraction}, publisher = {Hrvatsko dru\v{s}tvo kemijskih in\v{z}enjera i tehnologa (HDKI)}, publisherplace = {Rovinj, Hrvatska} }




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