Naslov
Extraction of vitamin E from hens feed mixture

Autori
Galović, Olivera ; Pritišanac, Ivan ; Dončić, Renata ; Košević, Manuela ; Kralik, Zlata ; Kralik, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
28th Croatian Meeting of Chemists and Chemical Engineers ; Book of Abstracts / Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023, 72-72

Skup
28th Croatian Meeting of Chemists and Chemical Engineers

Mjesto i datum
Rovinj, Hrvatska, 28.03.2023. - 31.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
vitamin E , extraction , hens feed mixture

Sažetak
Herbert Evans and Katherine Bishop discovered vitamin E in 1922. Vitamin E is one of the main antioxidants, slows down the development of Alzheimer's disease, protects the skin from unfavorable UV rays. Its positive effect on the human body ensured its significant place in nutrition. Humans cannot synthesize vitamin E, therefore it must be ingested through food. Foods rich in vegetable oils and green vegetables are considered a good source of vitamin E, while food of animal origin contains less vitamin E. Determination of vitamin E concentration in feed for laying hens is important because eggs are one of the sources of vitamin E in the human diet, and the concentration of vitamin E in egg yolk depends on the composition of the feed consumed by laying hens. A method developed by Pinheiro-Sant et al. was used for extraction of vitamin E from feed for laying hens. The influence of sample particle size, sample:extraction mixture ratio and optimizing the composition of the extraction mixture was investigated. All measurements were performed on a sample of laying hens feed available on the market, and the concentration of vitamin E was determined using HPLC. The determined concentration of vitamin E in the grinded and sieved samples was 266, 613 mg/kg, while in the non-grinded samples it was 199, 293 mg/kg, which is a difference of 33.78%. Sample:extraction mixture ratios (1:5, 1:10, 1:25 and 1:50) were investigated. The best determined ratio is 1:25 where the measured concentration of vitamin E was 329, 319 mg/kg. By replacing hexane:ethyl acetate (85:15, v/v) with hexane:ethyl acetate (65:35, v/v) better results were obtained. The measured concentration of vitamin E was 11.17% higher.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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