Naslov
Neuroprotection of mice exposed to nerve agent by acetylcholinesterase reactivator RS194B

Autori
Maček Hrvat, Nikolina ; Ilić, Katarina ; Kolić, Dora ; Taylor, Palmer ; Kalanj Bognar, Svjetlana ; Kovarik, Zrinka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Arhiv za higijenu rada i toksikologiju / - , 2023, A16-A16

Skup
Cell-Based Research in Toxicology and Drug Design

Mjesto i datum
Zagreb, Hrvatska, 26.01.2023

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Neuroprotection, organophosphate, neuroinflamation, oxime, GFAP, IBA-1, NeuN

Sažetak
Inhibition of the physiological function of acetylcholinesterase (AChE) by organophosphate (OP) molecules that readily cross the blood- brain barrier (BBB) promotes hypercholinergic activity which induces seizures leading to brain damage and neuroinflammation. OP-induced overstimulation of nicotinic and muscarinic membrane receptors in severe cases can lead to hypoxia, vasodepression, and respiratory arrest, followed by death. Currently approved therapy by permanently charged oxime compounds that don’t cross BBB readily is inadequate for restoring the activity of synaptic AChE, leaving the brain vulnerable to long-term damage. We anticipate that the treatment with uncharged, but ionizable oximes that cross the BBB and reactivate OP-inhibited synaptic AChE will act protectively on the brain of mice exposed to an organophosphorus nerve agent. For that purpose, a microglial response, detected with IBA-1 protein, glial cells, detected with glial fibrillary acidic protein (GFAP), and neuronal cell viability, detected by the neuronal nuclei antigen NeuN, were all monitored in order to indicate the survival of neurons and neuroprotection by RS194B oxime in the brain of mice exposed to a nerve agent.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA