Pregled bibliografske jedinice broj: 1262651
Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities
Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities // Biosensors, 13 (2023), 3; 13030404, 15 doi:10.3390/bios13030404 (međunarodna recenzija, pregledni rad, znanstveni)
CROSBI ID: 1262651 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Label-Free Long-Term Methods for Live Cell Imaging
of Neurons: New Opportunities
Autori
Baričević, Zrinko ; Ayar, Zahra ; Leitao, Samuel M. ; Mladinic, Miranda ; Fantner, Georg E. ; Ban, Jelena
Izvornik
Biosensors (2079-6374) 13
(2023), 3;
13030404, 15
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni
Ključne riječi
neuronal cultures ; live cell imaging ; label-free imaging ; phototoxicity ; scanning ion conductance microscopy ; atomic force microscopy ; digital holography microscopy
Sažetak
Time-lapse light microscopy combined with in vitro neuronal cultures has provided a significant contribution to the field of Developmental Neuroscience. The establishment of the neuronal polarity, i.e., formation of axons and dendrites, key structures responsible for inter- neuronal signaling, was described in 1988 by Dotti, Sullivan and Banker in a milestone paper that continues to be cited 30 years later. In the following decades, numerous fluorescently labeled tags and dyes were developed for live cell imaging, providing tremendous advancements in terms of resolution, acquisition speed and the ability to track specific cell structures. However, long-term recordings with fluorescence-based approaches remain challenging because of light-induced phototoxicity and/or interference of tags with cell physiology (e.g., perturbed cytoskeletal dynamics) resulting in compromised cell viability leading to cell death. Therefore, a label-free approach remains the most desirable method in long-term imaging of living neurons. In this paper we will focus on label-free high-resolution methods that can be successfully used over a prolonged period. We propose novel tools such as scanning ion conductance microscopy (SICM) or digital holography microscopy (DHM) that could provide new insights into live cell dynamics during neuronal development and regeneration after injury.
Izvorni jezik
Engleski
Znanstvena područja
Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-7060 - Istraživanje granice između neurodegeneracije i neuroregeneracije: identifikacija ključnih molekula pomoću proteomike i funkcionalnih testova na leđnoj moždini sisavaca (DefineREgenAgeMode) (Mladinić Pejatović, Miranda, HRZZ - 2016-06) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-prirod-18-290 1463 - Identifikacija i kvantifikacija neuralnih matičnih stanica novom metodom optimizirane homogenizacije (Ban, Jelena, NadSve - UNIRI projekti 2018) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-biomed-18-258 6427 - Otkrivanje ključnih molekula koje kontroliraju stanične mehanizme neuroprotekcije i neuroregeneracije posredovane proteinima toplinskog stresa nakon in vitro ozljede leđne moždine oposuma (Mladinić Pejatović, Miranda, NadSve - UNIRI projekti 2018) ( CroRIS)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE