Naslov
Entrapment of peptidoglycans and adamantyltripeptides into liposomes : an HPLC assay for determination of encapsulation efficiency

Autori
Frkanec, Ruža ; Travaš, Dijana ; Krstanović, Marina ; Halassy Špoljar, Beata ; Ljevaković, Đurđica ; Vranešić, Branka ; Frkanec, Leo ; Tomašić, Jelka

Izvornik
Journal of liposome research (0898-2104) 13 (2003), 3&4; 279-296

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
peptidoglycan monomer; adamantyltripeptides; liposomes; HPLC; encapsulation

Sažetak
The encapsulation of different immunomodulating peptides, the peptidoglycan monomer, its semisynthetic derivatives (Adamant-1-yl)-acetyl-peptidoglycan monomer and Boc-Tyr-peptidoglycan monomer, respectively, and of two diastereoisomers of adamantyltripeptides into the large negatively charged multilamellar liposomes was investigated. The reproducible quantitative method using HPLC was established for the determination of the entrapped compounds. It was shown that the tested compounds could be efficiently incorporated into liposomes using either the film or modified film method. The results confirmed that the peptidoglycans with lipophilic supstituents and particularly the adamantyltripeptides were incorporated into liposomes with higher efficiency than the peptidoglycan monomer using either of the described methods. Liposome preparations were stable at 4 °C up to seven days as shown by minimal leaking of the entrapped material.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA