Naslov
MIR-182-5P and MIR-375-3P as biomarkers for differentiating prostate cancer from benign prostate hyperplasia

Autori
Abramović, Irena ; Pezelj, Ivan ; Vrhovec, Borna ; Kuliš, Tomislav ; Vrtarić, Alen ; Ulamec, Monika ; Sinčić, Nino

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2022, 62-62

Skup
International congress of the Croatian society of biochemistry and molecular biology HDBMB22 "From science to knowledge"

Mjesto i datum
Brela, Hrvatska, 28.09.2022. - 01.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
prostate cancer ; biomarkers ; miRNAs

Sažetak
Prostate cancer (PCa) represents a malignancy with the highest prevalence and very high incidence among men worldwide. Clinical challenge is to differentiate localized PCa from benign prostate hyperplasia (BPH) due to the lack of specificity of routinely used biomarker PSA. Epigenetic biomarkers in liquid biopsies, especially miRNA, could address this challenge. The absolute expression of miR375-3p, miR-182-5p, miR-21-5p, and miR-148a-3p were quantified in blood plasma and seminal plasma of 65 PCa and 58 BPH patients by digital droplet PCR. The sensitivity and specificity of these microRNAs were determined using ROC curve analysis. The higher expression of miR-182- 5p and miR-375-3p in the blood plasma of PCa patients was statistically significant as compared to BPH (p = 0.0363 and 0.0226, respectively). Their combination achieved a specificity of 90.2 % for predicting positive or negative biopsy results, while PSA cut-off of 4 µg/L performed with only 1.7 % specificity. In seminal plasma, miR-375- 3p, miR-182-5p, and miR- 21-5p showed a statistically significantly higher expression in PCa patients with PSA >10 µg/L compared to ones with PSA >10 µg/L. MiR182-5p and miR-375-3p in blood plasma show higher performance than PSA in differentiating PCa from BPH. Seminal plasma requires further investigation as it represents an obvious source for PCa biomarker identification.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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