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Pregled bibliografske jedinice broj: 1261130

Pleomorphic Variants of Borreliella (syn. Borrelia) burgdorferi Express Evolutionary Distinct Transcriptomes


Čorak, Nina; Anniko, Sirli; Daschkin-Steinborn, Christina; Krey, Viktoria; Koska, Sara; Futo, Momir; Široki, Tin; Woichansky, Innokenty; Opašić, Luka; Kifer, Domagoj et al.
Pleomorphic Variants of Borreliella (syn. Borrelia) burgdorferi Express Evolutionary Distinct Transcriptomes // International journal of molecular sciences, 24 (2023), 6; 5594, 23 doi:10.3390/ijms24065594 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1261130 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Pleomorphic Variants of Borreliella (syn. Borrelia) burgdorferi Express Evolutionary Distinct Transcriptomes

Autori
Čorak, Nina ; Anniko, Sirli ; Daschkin-Steinborn, Christina ; Krey, Viktoria ; Koska, Sara ; Futo, Momir ; Široki, Tin ; Woichansky, Innokenty ; Opašić, Luka ; Kifer, Domagoj ; Tušar, Anja ; Maxeiner, Horst-Günter ; Domazet-Lošo, Mirjana ; Nicolaus, Carsten ; Domazet-Lošo, Tomislav

Izvornik
International journal of molecular sciences (1422-0067) 24 (2023), 6; 5594, 23

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
transcriptomes ; Borreliella ; Borrelia ; morphotypes ; RNAseq ; evolution ; pleomorphic variants ; phylostratigraphy ; orphan genes

Sažetak
Borreliella (syn. Borrelia) burgdorferi is a spirochete bacterium that causes tick-borne Lyme disease. Along its lifecycle B. burgdorferi develops several pleomorphic forms with unclear biological and medical relevance. Surprisingly, these morphotypes have never been compared at the global transcriptome level. To fill this void, we grew B. burgdorferi spirochete, round body, bleb, and biofilm- dominated cultures and recovered their transcriptomes by RNAseq profiling. We found that round bodies share similar expression profiles with spirochetes, despite their morphological differences. This sharply contrasts to blebs and biofilms that showed unique transcriptomes, profoundly distinct from spirochetes and round bodies. To better characterize differentially expressed genes in non-spirochete morphotypes, we performed functional, positional, and evolutionary enrichment analyses. Our results suggest that spirochete to round body transition relies on the delicate regulation of a relatively small number of highly conserved genes, which are located on the main chromosome and involved in translation. In contrast, spirochete to bleb or biofilm transition includes substantial reshaping of transcription profiles towards plasmids-residing and evolutionary young genes, which originated in the ancestor of Borreliaceae. Despite their abundance the function of these Borreliaceae-specific genes is largely unknown. However, many known Lyme disease virulence genes implicated in immune evasion and tissue adhesion originated in this evolutionary period. Taken together, these regularities point to the possibility that bleb and biofilm morphotypes might be important in the dissemination and persistence of B. burgdorferi inside the mammalian host. On the other hand, they prioritize the large pool of unstudied Borreliaceae-specific genes for functional characterization because this subset likely contains undiscovered Lyme disease pathogenesis genes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne tehničke znanosti, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-5924 - Filostratigrafija nastanka i gubitka gena (PhyLoss) (Domazet-Lošo, Tomislav, HRZZ - 2016-06) ( CroRIS)
EK-955626 - Inovativne strategije protiv bakterijskih infekcija (PEST-BIN) (Domazet-Lošo, Tomislav, EK ) ( CroRIS)
KK.01.1.1.01.0009 - Napredne metode i tehnologije u znanosti o podatcima i kooperativnim sustavima (EK )

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Fakultet elektrotehnike i računarstva, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Hrvatsko katoličko sveučilište, Zagreb

Poveznice na cjeloviti tekst rada:

doi doi.org www.mdpi.com

Citiraj ovu publikaciju:

Čorak, Nina; Anniko, Sirli; Daschkin-Steinborn, Christina; Krey, Viktoria; Koska, Sara; Futo, Momir; Široki, Tin; Woichansky, Innokenty; Opašić, Luka; Kifer, Domagoj et al.
Pleomorphic Variants of Borreliella (syn. Borrelia) burgdorferi Express Evolutionary Distinct Transcriptomes // International journal of molecular sciences, 24 (2023), 6; 5594, 23 doi:10.3390/ijms24065594 (međunarodna recenzija, članak, znanstveni)
Čorak, N., Anniko, S., Daschkin-Steinborn, C., Krey, V., Koska, S., Futo, M., Široki, T., Woichansky, I., Opašić, L. & Kifer, D. (2023) Pleomorphic Variants of Borreliella (syn. Borrelia) burgdorferi Express Evolutionary Distinct Transcriptomes. International journal of molecular sciences, 24 (6), 5594, 23 doi:10.3390/ijms24065594.
@article{article, author = {\v{C}orak, Nina and Anniko, Sirli and Daschkin-Steinborn, Christina and Krey, Viktoria and Koska, Sara and Futo, Momir and \v{S}iroki, Tin and Woichansky, Innokenty and Opa\v{s}i\'{c}, Luka and Kifer, Domagoj and Tu\v{s}ar, Anja and Maxeiner, Horst-G\"{u}nter and Domazet-Lo\v{s}o, Mirjana and Nicolaus, Carsten and Domazet-Lo\v{s}o, Tomislav}, year = {2023}, pages = {23}, DOI = {10.3390/ijms24065594}, chapter = {5594}, keywords = {transcriptomes, Borreliella, Borrelia, morphotypes, RNAseq, evolution, pleomorphic variants, phylostratigraphy, orphan genes}, journal = {International journal of molecular sciences}, doi = {10.3390/ijms24065594}, volume = {24}, number = {6}, issn = {1422-0067}, title = {Pleomorphic Variants of Borreliella (syn. Borrelia) burgdorferi Express Evolutionary Distinct Transcriptomes}, keyword = {transcriptomes, Borreliella, Borrelia, morphotypes, RNAseq, evolution, pleomorphic variants, phylostratigraphy, orphan genes}, chapternumber = {5594} }
@article{article, author = {\v{C}orak, Nina and Anniko, Sirli and Daschkin-Steinborn, Christina and Krey, Viktoria and Koska, Sara and Futo, Momir and \v{S}iroki, Tin and Woichansky, Innokenty and Opa\v{s}i\'{c}, Luka and Kifer, Domagoj and Tu\v{s}ar, Anja and Maxeiner, Horst-G\"{u}nter and Domazet-Lo\v{s}o, Mirjana and Nicolaus, Carsten and Domazet-Lo\v{s}o, Tomislav}, year = {2023}, pages = {23}, DOI = {10.3390/ijms24065594}, chapter = {5594}, keywords = {transcriptomes, Borreliella, Borrelia, morphotypes, RNAseq, evolution, pleomorphic variants, phylostratigraphy, orphan genes}, journal = {International journal of molecular sciences}, doi = {10.3390/ijms24065594}, volume = {24}, number = {6}, issn = {1422-0067}, title = {Pleomorphic Variants of Borreliella (syn. Borrelia) burgdorferi Express Evolutionary Distinct Transcriptomes}, keyword = {transcriptomes, Borreliella, Borrelia, morphotypes, RNAseq, evolution, pleomorphic variants, phylostratigraphy, orphan genes}, chapternumber = {5594} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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