Naslov
Influence of in vitro cultivation on differentiation gene expression in canine adipose- derived mesenchymal stem cells

Autori
Prišlin Marina, Vlahović Dunja, Ljolje Ivana, Kostešić Petar, Turk Nenad, Naletilić Šimun, Brnić Dragan, Krešić Nina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, pp prezentacija, znanstveni

Skup
1st European Congress on Medical and Veterinary engineering

Mjesto i datum
Zagreb, Hrvatska, 20.10.2022. - 22.10.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
canine adipose-derived mesenchymal stem cells ; differentiation ; gene expression ; in vitro cultivation ; microarray

Sažetak
Canine adipose-derived mesenchymal stem cells (cAD-MSCs) have been shown to be efficient in various pathologies, but certain therapies require in vitro expansion that can affect the molecular signatures and functionality of cAD-MSCs. Therefore, our research aimed to explore the existence of differ-entiation (DI) events in cAD- MSCs molecular signatures during in vitro cul- tivation. We isolated cAD-MSCs from the abdominal adipose tissue of 8 young fe-male dogs and confirmed their identity and functionality following the ISCT criteria. Good quality total RNA extracted in passage (P) 3 and P6 was used in a microarray and changes in the expression of genes related to DI were analyzed. Cells arrested proliferation at ~P8 and demonstrated common immunophe-notype and DI peculiarities, except for lower-intensity osteoDI. Overall ex-pression was not significantly changed, although gene upregulations and downregulations were noticed. Overexpression of GDF7, important in carti-lage formation, KDR or VEGFR2 in angiogenesis during DI, and FGF10, in development, especially in embryonic limb development, indicate that fur-ther in vitro cultivation could favor DI events. TBX5, known as the promot-er of osteoDI and mineralization was downregulated in P6, which suggests its possible impact on the demonstrated decreased potential for osteoDI. Downregulation of RHOA is a beneficial finding since overexpression of this gene is associated with tumor cell proliferation and metastasis. This research demonstrated that in vitro exploitation does not significantly alter the DI potential in P3 nor DI gene expression in P6 in young female cAD-MSCs, suggesting the absence of significant spontaneous DI events during cultivation. Preserved multipotent potential during cultivation, as demonstrated here, is a crucial prerequisite for the generation of good- quality stem cell products for canines.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

Napomena
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