Pregled bibliografske jedinice broj: 1260827
Human Decidual CD1a+ Dendritic Cells Undergo Functional Maturation Program Mediated by Gp96
Human Decidual CD1a+ Dendritic Cells Undergo Functional Maturation Program Mediated by Gp96 // International journal of molecular sciences, 24 (2023), 3; 2278-2293 doi:10.3390/ijms24032278 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1260827 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Human Decidual CD1a+ Dendritic Cells Undergo Functional Maturation Program Mediated by Gp96
Autori
Gulić, Tamara ; Laskarin, Gordana ; Glavan, Lana ; Grubić Kezele, Tanja ; Haller, Herman ; Rukavina, Daniel.
Izvornik
International journal of molecular sciences (1422-0067) 24
(2023), 3;
2278-2293
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
decidual dendritic cells ; chemokine ; cytokine ; gp96 ; pregnancy
Sažetak
Heat shock proteins (hsps), in certain circumstances, could shape unique features of decidual dendritic cells (DCs) that play a key role in inducing immunity as well as maintaining tolerance. The aim of the study was to assess the binding of gp96 to Toll-like receptor (TLR) 4 and CD91 receptors on decidual CD1a+ DCs present at the maternal-fetal interface in vitro as well as the influence of CD1a+ DCs maturation status. Immunohistology and immunofluorescence of paraffin-embedded first-trimester decidua tissue sections of normal and pathological (missed abortion MA and blighted ovum BO) pregnancies were performed together with flow cytometry detection of antigens in CD1a+ DCs after gp96 stimulation of decidual mononuclear cells. Gp96 efficiently bound CD91 and TLR4 receptors on decidual CD1a+ DCs in a dose-dependent manner and increased the expression of CD83 and HLA-DR. The highest concentration of gp96 (1000 ng/mL) increased the percentage of Interferon-γ (INF-γ) and IL-15 expressing gp96+ cells. Gp96 binds CD91 and TLR4 on decidual CD1a+ DCs, which causes their maturation and significantly increases INF-γ and IL-15 in the context of Th1 cytokine/chemokine domination, which could support immune response harmful for ongoing pregnancy.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Ustanove:
Medicinski fakultet, Rijeka,
Klinički bolnički centar Rijeka,
Sveučilište u Rijeci
Profili:
Tamara Gulić
(autor)
Herman Haller
(autor)
Tanja Grubić Kezele
(autor)
Daniel Rukavina
(autor)
Gordana Laškarin
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE