Naslov
Antiulcer BPC 157 effect in 96% alcohol instilled-rats counteracts increased intracranial pressure, portal and caval hypertension and aortal hypotension, along with both venous and arterial cloth formation

Autori
Gojković, Slaven ; Krezić, Ivan ; Žižek, Helena ; Malekinušić, Dominik ; Đurašin, Tajana ; Vraneš, Hrvoje ; Drmić, Domagoj ; Dretar, Vilim ; Horvat, Katarina ; Škrtić, Anita ; Boban Blagaić, Alenka ; Seiwerth, Sven ; Sikirić, Predrag

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Gastroenterology (New York, N.Y. 1943), 158 (2020), 6(S) / - , 2020, S-501

Skup
Digestive Disease Week

Mjesto i datum
Online; konferencija, 02.05.2020. - 05.05.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
gastric ulcer ; alcohol ; BPC 157

Sažetak
Aim. We hypothesized that, in addition to the severe gastric lesions, the Robert’s classic instillation of the 96% alcohol in the rat stomach may induce increased intracranial pressure, portal and caval hypertension and aortal hypotension, along with both venous and arterial cloth formation, which may be counteracted by the stable gastric pentadecapeptide BPC AGA Abstracts AGA Abstracts 157 therapy. Namely, BPC 157, as new mediator of Robert’s cytoprotection, counteracts both acute and chronic alcohol gastric lesions, much like acute and chronic alcohol intoxication. BPC 157 counteracted bile duct ligation-induced liver cirrhosis and portal hypertension (Eur J Pharmacol 2019 ; 847:130-142), as well as attenuated liver and gastric lesions and eliminated portal hypertension in chronically alcohol drinking rats (Curr Pharm Des 2018 ; 24:1990-2001). In rats with inferior caval vein occlusion, BPC 157 counteracts venous hypertension and aortal hypotension, and thrombosis formation (Vascul Pharmacol 2018 ; 106:54-66). Methods. To evaluate intracranial pressure in anesthetized rats, we made a single burr hole in the sagittal suture rostral part, above the superior sagittal sinus, cannulation of the sinus anterior part by Braun intravenous cannules for intravascular pressure measurement before and at 5 min after instillation of the 96% alcohol into the rat stomach and medication application (BPC 157 10µg, 10ng/kg, or saline, 1 ml/rat given intraperitoneally) (Fig. C/B). Portal and caval hypertension and aortal hypotension and thrombosis were assessed along with hemorrhagic gastric lesions presentation as described (Vascul Pharmacol 2018 ; 106:54-66). Results. Healthy rats exhibit: superior sagittal sinus pressure between -26 and -28 mmHg ; portal pressure between 3 and 5 mm Hg or like the pressure in the inferior caval vein (providing at least 1 mm Hg higher values in the portal vein) ; abdominal aorta blood pressure values between 100 and 120 mm Hg at the level of bifurcation. BPC 157 counteracted considerably increased pressure values in the superior sagittal sinus (25±2 mmHg (controls) vs. -24±2 (µg), -26±2 (ng)). There were also counteracted portal hypertension (24±2 mmHg (controls) vs. 5±1 (µg), 6±2 (ng)), caval hypertension (14±2 mmHg (controls) vs. 6±2 (µg), 7±2 (ng)) and aortal hypotension (81±3 mmHg (controls) vs. 100±2 (µg) , 105±5 (ng)). Accordingly, thrombosis was markedly attenuated (superior mesenteric vein 0.0133±0.0008 g (controls) vs. 0.0092±0.0005 (µg), 0.0089±0.0008 (ng)) and artery 0.0048±0.0006 g (controls) vs. 0.0005±0.0002 (µg), 0.0007±0.0003 (ng)). Gastric lesions (as % of total stomach glandular area) cover 15±2% (control) vs. 5±2% (µg), 6±2% (ng). Conclusion. Antiulcer BPC 157 effect in 96% alcohol instilled-rats counteracts increased intracranial pressure, portal and caval hypertension and aortal hypotension, along with both venous and arterial cloth formation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA