Naslov
MITOCHONDRIAL REACTIVE OXYGEN SPECIES DRIVE REPROGRAMMING OF MESOTHELIOMA CELL LINE

Autori
Sikirić, Sunčana ; Šepac, Ana ; Cindrić, Marina ; Seiwerth, Fran ; Milavić, Marija ; Batelja Vuletić, Lovorka ; Sedlić, Filip

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th Anniversary of Targeting Mitochondria / - , 2019, 157-157

Skup
10th Anniversary of Targeting Mitochondria Congress

Mjesto i datum
Berlin, Njemačka, 28.10.2019. - 29.10.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mitochondria ; ROS ; reprogramming ; mesothelioma

Sažetak
Introduction: The expression of pluripotency genes, such as OCT4, NANOG and SOX2 is associated with dedifferentiation of cancer and poor prognosis of cancer patients (1, 2). We investigated whether human malignant mesothelioma cell line Mero-14 expresses pluripotency genes, and whether their expression is affected by reactive oxygen species (ROS) produced by mitochondria. Materials & Methods: The expression of OCT4, NANOG and SOX2, and control genes found in mesothelial cells, vimentin and cytokeratin 7 was analyzed by DAB immunohistochemistry. ROS generation was analyzed using CM-H2DCFDA fluorescence indicator and Evos imaging system. Complex III inhibitor antimycin A was used to stimulate mitochondrial ROS generation, while mitoTEMPO was used to scavenge ROS generated by mitochondria. Results: Mero-14 cells exhibited expression NANOG and SOX2 and no expression of OCT4. Complex III inhibitor antimycin A dose- dependently increased mitochondrial ROS generation. At lower, but not higher concentrations antimycin A enhanced NANOG expression, but did not affect SOX2 expression or expression of vimentin and cytokeratin 7 that were also expressed in Mero-14 cells. mitoTEMPO abrogated antimycin A-induced increase in NANOG expression. Conclusion: Mero-14 cells express pluripotency genes NANOG and SOX2. ROS generated by mitochondria induce NANOG expression and therefore may trigger reprogramming of mesothelioma cells toward malignant phenotypes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA