Naslov
Pentadecapeptide BPC 157 After 70% Liver Resection in Rats

Autori
Dalibor Crvenkovic, Marko Sever, Anita Zenko Sever, Domagoj Drmic, Igor Petrovic, Zeljko Romic, Sven Seiwerth, Predrag Sikiric

Izvornik
Gastroenterology (New York, N.Y. 1943) (0016-5085) (2015), 1; 964-964

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, stručni

Ključne riječi
pentadecapeptide BPC 157, liver resection, paracetamol

Sažetak
Aim We suggest that stable gastric pentadecapeptide BPC 157 improves function and liver regeneration after 70% liver resection in rats since it can rescue otherwise severe liver lesions after overdose of paracetamol, diclofenac, ibuprofen, insulin, CCl4 or chronic alcohol abuse (for review see, i.e., Curr Pharm Des. 2012). Materials and methods In male Wistar rats, 70 % liver resection was performed . BPC 157 was applied (10 μg, 10 ng) (/kg) either ip once daily or in drinking water (0.16 μg ; 0.16 ng/ml/12ml/day) till the sacrifice (at day 3, 7, 14, 21, 28). Macroscopic observation, weight (animal(g) and liver weight/animal weight ratio), microscopical observation (binuclear hepatocyte number, hepatocyte nuclei diameter, hepatocyte area) and biochemical analasis (AST, ALT, serum bilirubine level) were performed. Results BPC 157-rats maintained the weight, exhibited better liver regeneration based on better liver mass/body weight ratio (i.e., 14 days: 0.037 +/-0.004 (BPC 157) vs. 0.023+/- 0.005 (control) ; 28 days: 0.053+/-0.007 (BPC 157) vs. 0.03 +/- 0.004 (control)) and larger liver volume in pentadecapeptide BPC 157-rats. Unlike constantly increased AST, ALT, bilirubine levels in controls, BPC 157-rats after a short post-surgery increase, mostly presented values comparable to non-resected animals (conforming functional liver regeneration). Micro- scopically, controls presented larger areas of liver steatosis focal necrosis and liver fibrosis unlike BPC 157-rats. Although binuclear cells were sporadically seen in controls, there were less hepatocytes with mitosis compared to all BPC 157 regimens. Conclusions BPC 157- rats maintained the weight, exhibited better liver regeneration. Unlike constantly increased AST, ALT, bilirubine levels in controls, BPC 157- rats mostly presented serum values compara- ble to non-resected animals. Key words: BPC 157, liver resection, rats

Izvorni jezik
Engleski

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