Naslov
BPC 157 Rescues NO‐System in Perforated Stomach and Caecum

Autori
Drmic, Domagoj ; Vidovic, Tinka ; Vlainic, Josipa ; Samara, Mariam ; Gojkovic, Slaven ; Krezic, Ivan ; Jeyakumar, Jerusha ; Bilic, Zdenko ; Seiwerth, Sven ; Sikiric, Predrag

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FASEB Journal. 2018 ; 32(S1) / - : John Wiley & Sons, 2018, 83212-83212

Skup
Experimental Biology

Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 21.04.2018. - 25.04.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
BPC 157 ; NO system, perforated stomach

Sažetak
Aim We focused on perforated defect in stomach and caecum, and NO-tissue levels in rats and the therapy with the stable gastric pentadecapeptide BPC 157. Effective in perforated stomach lesion and perforated caecum lesion as well as ischemic colitis, ischemia and reperfusion, BPC 157 rapidly activates vessels “running” toward the perforated defect (stomach perforation (Gastroenterology 2017 ; 152, Issue 5, Supplement 1, S889–S890 ; Gastroenterology 2017 ; 152, Issue 5, Supplement 1, S890–S891) or to bypass vessel obstruction (ischemic colitis)(World Journal of Gastroenterology, in press, 2017) and largely attenuates lesions course. Methods At 15 minutes post-injury, we determined nitric oxide (NO) levels in stomach caecum tissue samples using the Griess reaction (Griess Reagent System, Promega, USA). Sulfanilamide was added to the homogenized tissue, incubated and then N-1- naphthylethylenediamine dihydrochloride was added. The Griess reaction is based on diazotization reaction in which acidified nitrite reacts with diazonium ions and in a further step are coupled to N-1-naphthylethylenediamine dihydrochloride forming chromophoric azo derivate. Absorbance was measured at 540 nm, using sodium nitrite solution as standard. NO levels are reported in μmol/mg protein. Proteins were determined using the commercial kit (BioRad Protein DR Assay Reagent Kit, USA). Medication was bath (BPC 157 10 ug, 10 ng/kg/1ml bath/rat ; equivolume saline (controls)) at the ligated perforated injury, in stomach or caecum, at 1 min after perforated injury induction. Results Stomach. In the early term, we noted fewer NO- levels in rats underwent stomach perforation, but a reversal with BPC 157 administration, and thereby, NO levels like in the healthy appear in rats with perforated stomach underwent BPC 157 medication (Fig. 1). Caecum. Likewise, NO level assessment demonstrated a drop in level in rats which underwent caecum perforation, but the NO values in rats with perforated caecum which underwent BPC 157 medication were similar to the NO-levels in healthy rats (Fig. 2). Conclusions Rapid activation of vessels “running” toward the perforated defect (perforated defect healing and attenuated bleeding, counteraction of free radicals formation previously described by BPC 157 application, occurs with the parallel rescue of the NO-system in the both of the perforated tissues (stomach perforation (Gastroenterology 2017 ; 152, Issue 5, Supplement 1, S889–S890 ; caecum perforation (Gastroenterology 2017 ; 152, Issue 5, Supplement 1, S890–S891)).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne tehničke znanosti

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