Naslov
DNA METHYLATION PROFILE IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE FROM TWO DIFFERENT REGIONS IN CROATIA.

Autori
Jukić, Marijana ; Basioli Kasap, Eugenija ; Jelavić, Ivana ; Škrinjarić Cincar, Sanda ; and Glavaš- Obrovac, Ljubica.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDBMB22: From Science to Knowledge / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana. - Zagreb, 2022, 88-88

Skup
International Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: FROM SCIENCE TO KNOWLEDGE

Mjesto i datum
Brela, Croatia, 28.09.2022. - 01.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
COPD, DNA methylation status

Sažetak
Chronic obstructive pulmonary disease (COPD) is a third leading cause of death worldwide, presenting as an abnormal inflammatory response of the lungs to inhaled noxious particles or gases. The influence of tobacco smoke can lead to epigenetic changes such as DNA methylation that represents a reversible regulatory gene modification. Previous studies has shown that global DNA methylation could be useful as a biomarker in COPD. The goal of this work was to investigate if there is a difference in the global DNA methylation between the control group and the group of COPD patients from Osijek and Zadar respected to different climate and lifestyle. Based on the calculation of the mean effect in different numerical variants between the two independent groups, 136 patients with COPD, 69 patients treated in Clinical Hospital Center Osijek, and 67 patients treated in General Hospital Zadar, were included in this research. The control group consists of 64 subjects, 32 from each center. DNA for global DNA methylation measurement was isolated from leukocytes using the commercial set NucleoSpin Blood L (Macherey Nagel, Germany), and after that absolute quantification of global DNA methylation was done with colorimetric method by commercial set Methylated DNA Quantification Kit (Colorimetic) (ab117128) Abcam (Cambridge, United Kingdom) according to the manufacturer's instructions. The obtained results were analyzed using various statistical methods. The COPD patients were found to have lower values of global DNA methylation than the control group, but a satistically significant difference was found only between male patients with COPD and male control subjects in Zadar. Further research on global DNA methylation on a larger sample of healthy and COPD subjects from both observed regions should be done.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA