Pregled bibliografske jedinice broj: 1260196
Modulation of Notch1 signaling regulates bone fracture healing
Modulation of Notch1 signaling regulates bone fracture healing // Journal of Orthopaedic Research, 38 (2020), 11; 2350-2361 doi:10.1002/jor.24650 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1260196 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Modulation of Notch1 signaling regulates bone
fracture healing
Autori
Novak, Sanja ; Roeder, Emilie ; Sinder, Benjamin P. ; Adams, Douglas J. ; Siebel, Chris W. ; Grcevic, Danka ; Hankenson, Kurt D. ; Matthews, Brya G. ; Kalajzic, Ivo
Izvornik
Journal of Orthopaedic Research (0736-0266) 38
(2020), 11;
2350-2361
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Notch signaling ; alpha-smooth muscle actin ; bone fracture ; inducible Cre ; osteoblast differentiation ; periosteal progenitors
Sažetak
Fracture healing involves interactions of different cell types, driven by various growth factors, and signaling cascades. Periosteal mesenchymal progenitor cells give rise to the majority of osteoblasts and chondrocytes in a fracture callus. Notch signaling has emerged as an important regulator of skeletal cell proliferation and differentiation. We investigated the effects of Notch signaling during the fracture healing process. Increased Notch signaling in osteochondroprogenitor cells driven by overexpression of Notch1 intracellular domain (NICD1) (αSMACreERT2 mice crossed with Rosa-NICD1) during fracture resulted in less cartilage, more mineralized callus tissue, and stronger and stiffer bones after 3 weeks. Periosteal cells overexpressing NICD1 showed increased proliferation and migration in vitro. In vivo data confirmed that increased Notch1 signaling caused expansion of alpha-smooth muscle actin (αSMA)- positive cells and their progeny including αSMA- derived osteoblasts in the callus without affecting osteoclast numbers. In contrast, anti- NRR1 antibody treatment to inhibit Notch1 signaling resulted in increased callus cartilage area, reduced callus bone mass, and reduced biomechanical strength. Our study shows a positive effect of induced Notch1 signaling on the fracture healing process, suggesting that stimulating the Notch pathway could be beneficial for fracture repair.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Ustanove:
Medicinski fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE