Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1260196

Modulation of Notch1 signaling regulates bone fracture healing


Novak, Sanja; Roeder, Emilie; Sinder, Benjamin P.; Adams, Douglas J.; Siebel, Chris W.; Grcevic, Danka; Hankenson, Kurt D.; Matthews, Brya G.; Kalajzic, Ivo
Modulation of Notch1 signaling regulates bone fracture healing // Journal of Orthopaedic Research, 38 (2020), 11; 2350-2361 doi:10.1002/jor.24650 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1260196 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Modulation of Notch1 signaling regulates bone fracture healing

Autori
Novak, Sanja ; Roeder, Emilie ; Sinder, Benjamin P. ; Adams, Douglas J. ; Siebel, Chris W. ; Grcevic, Danka ; Hankenson, Kurt D. ; Matthews, Brya G. ; Kalajzic, Ivo

Izvornik
Journal of Orthopaedic Research (0736-0266) 38 (2020), 11; 2350-2361

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Notch signaling ; alpha-smooth muscle actin ; bone fracture ; inducible Cre ; osteoblast differentiation ; periosteal progenitors

Sažetak
Fracture healing involves interactions of different cell types, driven by various growth factors, and signaling cascades. Periosteal mesenchymal progenitor cells give rise to the majority of osteoblasts and chondrocytes in a fracture callus. Notch signaling has emerged as an important regulator of skeletal cell proliferation and differentiation. We investigated the effects of Notch signaling during the fracture healing process. Increased Notch signaling in osteochondroprogenitor cells driven by overexpression of Notch1 intracellular domain (NICD1) (αSMACreERT2 mice crossed with Rosa-NICD1) during fracture resulted in less cartilage, more mineralized callus tissue, and stronger and stiffer bones after 3 weeks. Periosteal cells overexpressing NICD1 showed increased proliferation and migration in vitro. In vivo data confirmed that increased Notch1 signaling caused expansion of alpha-smooth muscle actin (αSMA)- positive cells and their progeny including αSMA- derived osteoblasts in the callus without affecting osteoclast numbers. In contrast, anti- NRR1 antibody treatment to inhibit Notch1 signaling resulted in increased callus cartilage area, reduced callus bone mass, and reduced biomechanical strength. Our study shows a positive effect of induced Notch1 signaling on the fracture healing process, suggesting that stimulating the Notch pathway could be beneficial for fracture repair.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Ivo Kalajzić (autor)

Avatar Url Sanja Novak (autor)

Avatar Url Danka Grčević (autor)

Poveznice na cjeloviti tekst rada:

doi onlinelibrary.wiley.com

Citiraj ovu publikaciju:

Novak, Sanja; Roeder, Emilie; Sinder, Benjamin P.; Adams, Douglas J.; Siebel, Chris W.; Grcevic, Danka; Hankenson, Kurt D.; Matthews, Brya G.; Kalajzic, Ivo
Modulation of Notch1 signaling regulates bone fracture healing // Journal of Orthopaedic Research, 38 (2020), 11; 2350-2361 doi:10.1002/jor.24650 (međunarodna recenzija, članak, znanstveni)
Novak, S., Roeder, E., Sinder, B., Adams, D., Siebel, C., Grcevic, D., Hankenson, K., Matthews, B. & Kalajzic, I. (2020) Modulation of Notch1 signaling regulates bone fracture healing. Journal of Orthopaedic Research, 38 (11), 2350-2361 doi:10.1002/jor.24650.
@article{article, author = {Novak, Sanja and Roeder, Emilie and Sinder, Benjamin P. and Adams, Douglas J. and Siebel, Chris W. and Grcevic, Danka and Hankenson, Kurt D. and Matthews, Brya G. and Kalajzic, Ivo}, year = {2020}, pages = {2350-2361}, DOI = {10.1002/jor.24650}, keywords = {Notch signaling, alpha-smooth muscle actin, bone fracture, inducible Cre, osteoblast differentiation, periosteal progenitors}, journal = {Journal of Orthopaedic Research}, doi = {10.1002/jor.24650}, volume = {38}, number = {11}, issn = {0736-0266}, title = {Modulation of Notch1 signaling regulates bone fracture healing}, keyword = {Notch signaling, alpha-smooth muscle actin, bone fracture, inducible Cre, osteoblast differentiation, periosteal progenitors} }
@article{article, author = {Novak, Sanja and Roeder, Emilie and Sinder, Benjamin P. and Adams, Douglas J. and Siebel, Chris W. and Grcevic, Danka and Hankenson, Kurt D. and Matthews, Brya G. and Kalajzic, Ivo}, year = {2020}, pages = {2350-2361}, DOI = {10.1002/jor.24650}, keywords = {Notch signaling, alpha-smooth muscle actin, bone fracture, inducible Cre, osteoblast differentiation, periosteal progenitors}, journal = {Journal of Orthopaedic Research}, doi = {10.1002/jor.24650}, volume = {38}, number = {11}, issn = {0736-0266}, title = {Modulation of Notch1 signaling regulates bone fracture healing}, keyword = {Notch signaling, alpha-smooth muscle actin, bone fracture, inducible Cre, osteoblast differentiation, periosteal progenitors} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font