Pregled bibliografske jedinice broj: 1259986
Anti-D reagents should be chosen accordingly to the prevalence of D variants in the obstetric population
Anti-D reagents should be chosen accordingly to the prevalence of D variants in the obstetric population // Journal of Clinical Laboratory Analysis, 32 (2018), 3; e22285, 5 doi:10.1002/jcla.22285 (međunarodna recenzija, članak, znanstveni)
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Naslov
Anti-D reagents should be chosen accordingly to the prevalence of D variants in the obstetric population
Autori
Lukacevic Krstic, Jelena ; Dajak, Slavica ; Bingulac-Popovic, Jasna ; Dogic, Vesna ; Mratinovic-Mikulandra, Jela
Izvornik
Journal of Clinical Laboratory Analysis (0887-8013) 32
(2018), 3;
E22285, 5
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
anti-D reagents ; D variants ; obstetric ; population
Sažetak
Background: Resolving ambiguous results of D antigen typing is crucial for appropriate and rational administration of anti-D immunoprophylaxis and transfusion practice in obstetric population. The aim of the study was to establish selection criteria of anti-D reagents for our population. Methods: A total of 12 689 samples from primiparous women in Split-Dalmatia County, Croatia, were typed for RhD antigen during the period of 5 years. Ambiguous results were submitted to additional serologic investigation and genotyping. RHD genotyping was performed by commercial genotyping kits (Ready Gene weak D ®and Ready gene CDE, Inno-Train, Kronberg, Germany). Relative frequencies and accompanying 95% confidence intervals were used to estimate the prevalence of variants. Results: The prevalence of D variants was 0.42% (95% CI 0.31 ; 0.53). The most common partial D variant was D Va (RHD*05.05), with the prevalence of 0.08% (95% CI 0.03 ; 0.13). All weak D variants were weak D types 1, 2 and 3 (RHD*weak D type 1, RHD*weak D type 2, RHD*weak D type 3). Weak D samples were distinguishable from partial D in routine typing due to the difference in reactivity of partial D samples with clones D7B8 and RUM-1. Cell line RUM-1 gives weak or negative reactions with partial DVa category. Conclusion: The most common partial D variant in our population is DVa. It is recommended to use cell lines which do not strongly agglutinate DVa variant in routine RhD typing. The appropriate choice of reagents will enable the serology methods to recognize the cases in which RHD genotyping is required.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Ustanove:
KBC Split,
Hrvatski zavod za transfuzijsku medicinu,
Medicinski fakultet, Split,
Sveučilište u Splitu Sveučilišni odjel zdravstvenih studija
Profili:
Jelena Lukačević Krstić
(autor)
Jasna Bingulac-Popović
(autor)
Jela Mratinović-Mikulandra
(autor)
Slavica Dajak
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE