Naslov
Confocal fluorescence microscopy of the plant cytoskeleton

Autori
Weber, Igor

Izvornik
Periodicum biologorum (0031-5362) 105 (2003), 3; 237-249

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
microtubules; microfilaments; actin; tubulin; cytokinesis; cell growth

Sažetak
Confocal laser scanning microscopy has become a widespread method for examination of the supramolecular structure in fluorescently labeled cells. Its ability to substantially reduce out-of-focus blur provides a significant improvement in resolution along the optical axis of a microscope and enables optical sectioning of thick specimens. These features are particularly helpful for investigation of plant cells and tissues, which have been difficult to analyze at high resolution by traditional microscopy because of their shape, size, and optical properties. I will review the contributions that confocal microscopy has made to the study of the plant cytoskeleton. The operational principle of confocal microscopy will be introduced, including a discussion of recent technical advances relevant for investigation of plant cells. A number of representative studies of both fixed and live plant cells will be referred to, in which confocal microscopy has provided new and much clearer information about cytoskeletal structure than has been possible to obtain with conventional wide-field fluorescence microscopy. Finally, special attention will be given to visualization of microtubules and actin microfilaments in growth and division of plant cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA