Naslov
CfDNA methylation in liquid biopsies as potential testicular seminoma biomarker

Autori
Raos, Dora ; Oršolić, Davor ; Mašić, Silvija ; Tomić, Miroslav ; Krasić, Jure ; Tomašković, Igor ; Gabaj, Nora Nikolac ; Gelo, Nina ; Kaštelan, Željko ; Kuliš, Tomislav ; Bojanac, Ana Katušić ; Barešić, Anja ; Ulamec, Monika ; Ježek, Davor ; Sincic, Nino

Izvornik
Epigenomics (1750-1911) 14 (2022), 23; 1493-1507

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA methylation ; biomarkers ; cfDNA ; liquid biopsy ; seminoma

Sažetak
Background: Seminoma is a testicular tumor type, routinely diagnosed after orchidectomy. As cfDNA represents a source of minimally invasive seminoma patient management, this study aimed to investigate whether cfDNA methylation of six genes from liquid biopsies, have potential as novel seminoma biomarkers. Materials & methods: cfDNA methylation from liquid biopsies was assessed by pyrosequencing and compared with healthy volunteers' samples. Results: Detailed analysis revealed specific CpGs as possible seminoma biomarkers, but receiver operating characteristic curve analysis showed modest diagnostic performance. In an analysis of panels of statistically significant CpGs, two DNA methylation panels emerged as potential seminoma screening panels, one in blood CpG8/CpG9/CpG10 (KITLG) and the other in seminal plasma CpG1(MAGEC2)/CpG1(OCT3/4). Conclusion: The presented data promote the development of liquid biopsy epigenetic biomarkers in the screening of seminoma patients.

Izvorni jezik
Hrvatski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti

POVEZANOST RADA