Naslov
Characterization of Atrazine Degrading Bacterial Communities
(Characterization of atrazine degrading bacterial communities)

Autori
Hršak, Dubravka ; Udiković, Nikolina ; Filipčić, Daša ; Martin Laurent, Fabrice

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book : 1st FEMS Congress of European microbiologists / Avšič-Županac ; van Belkum ; Bruschi ; Chet ; Cole ; Farr - Delft : Elsevier, 2003, 390-390

Skup
1st FEMS Congress of European microbiologists

Mjesto i datum
Ljubljana, Slovenija, 29.06.-03.07

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Atrazine degrading bacterial community; Atrazine mineralization; Microbial interactions
(atrazine degrading bacterial community; atrazine mineralization; microbial interactions)

Sažetak
The objective of this study was to evaluate biotransformation activity of atrazine degrading bacterial communities originating from aquatic and soil ecosystems, especially to assess their effectiveness for treatment of effluents from atrazine production. The enrichment of atrazine degrading bacteria was carried out in continuous-flow units under the inflow of mineral salts medium (AMS) with atrazine as only carbon and nitrogen source. Three communities (two enriched from effluents and one from soil of the herbicide factory) were selected as the most efficient since they showed complete disappearance of atrazine under continuous cultivation (monitored by HPLC analyses), as well as substantial mineralizing activity during batch cultivation in sealed flasks (60-70% atrazine released as carbon dioxide, determined by TIC analyses). Plating on selective AMS-agar plates suggested that during continuous cultivation the community structure changed significantly, with appearance of morphologically new strains. From these associations five strains were isolated expressing different atrazine mineralizing activity (17-53%). All these strains were capable to transform atrazine to cyanuric acid, but none of them could continue degradation, i.e. the cleavage of s-triazine ring. The capacity of isolated strains to degrade atrazine was further tested to the occurrence of known degrading genes. Enrichment of stable, structurally new bacterial communities which were more efficient in atrazine degradation than any of the individual populations alone suggested that the relationships between community members, including those based on combined metabolic capability and the transfer of catabolic genes, are important for successful atrazine mineralization.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA