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Pregled bibliografske jedinice broj: 1258016

SPRTN-dependent DPC degradation precedes repair of damaged DNA: a proof of concept revealed by the STAR assay


Glumac, Mateo; Polović, Mirjana; Batel, Anja; Gelemanović, Andrea; Maček, Boris; Velić, Ana; Marinović-Terzić, Ivana
SPRTN-dependent DPC degradation precedes repair of damaged DNA: a proof of concept revealed by the STAR assay // Nucleic Acids Research, (2023), 1-18 doi:10.1093/nar/gkad022 (recenziran, članak, znanstveni)


CROSBI ID: 1258016 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
SPRTN-dependent DPC degradation precedes repair of damaged DNA: a proof of concept revealed by the STAR assay

Autori
Glumac, Mateo ; Polović, Mirjana ; Batel, Anja ; Gelemanović, Andrea ; Maček, Boris ; Velić, Ana ; Marinović-Terzić, Ivana

Izvornik
Nucleic Acids Research (0305-1048) (2023); 1-18

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DPC isolation, STAR assay, SPRTN, DNA repair, H2Ax signaling, formaldehyde, topoisomerase inhibitor

Sažetak
DNA-protein crosslinks (DPCs), formed by the covalent conjugation of proteins to DNA, are toxic lesions that interfere with DNA metabolic processing and transcription. The development of an accurate biochemical assay for DPC isolation is a priority for the mechanistic understanding of their repair. Here, we propose the STAR assay for the direct quantification of DPCs, sensitive to physiologically relevant treatment conditions. Implementing the STAR assay revealed the formation of small cross-linked peptides on DNA, created by the proteolytic degradation of DPCs by SPRTN. The initial proteolytic degradation of DPCs is required for the downstream activation of DNA repair, which is mediated through the phosphorylation of H2Ax. This leads to the accumulation of DNA repair factors on chromatin and the subsequent complete removal of the cross- linked peptides. These results confirmed that the repair of DPCs is a two-step process, starting with proteolytic resection by SPRTN, followed by the repair of the underlying damage to the DNA.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-3097 - Uloga proteina Spartan u DNA replikaciji (SprtnRep) (Marinović Terzić, Ivana, HRZZ - 2016-06) ( CroRIS)

Ustanove:
Medicinski fakultet, Split,
Mediteranski institut za istraživanje života

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi academic.oup.com

Citiraj ovu publikaciju:

Glumac, Mateo; Polović, Mirjana; Batel, Anja; Gelemanović, Andrea; Maček, Boris; Velić, Ana; Marinović-Terzić, Ivana
SPRTN-dependent DPC degradation precedes repair of damaged DNA: a proof of concept revealed by the STAR assay // Nucleic Acids Research, (2023), 1-18 doi:10.1093/nar/gkad022 (recenziran, članak, znanstveni)
Glumac, M., Polović, M., Batel, A., Gelemanović, A., Maček, B., Velić, A. & Marinović-Terzić, I. (2023) SPRTN-dependent DPC degradation precedes repair of damaged DNA: a proof of concept revealed by the STAR assay. Nucleic Acids Research,, 1-18 doi:10.1093/nar/gkad022.
@article{article, author = {Glumac, Mateo and Polovi\'{c}, Mirjana and Batel, Anja and Gelemanovi\'{c}, Andrea and Ma\v{c}ek, Boris and Veli\'{c}, Ana and Marinovi\'{c}-Terzi\'{c}, Ivana}, year = {2023}, pages = {1-18}, DOI = {10.1093/nar/gkad022}, keywords = {DPC isolation, STAR assay, SPRTN, DNA repair, H2Ax signaling, formaldehyde, topoisomerase inhibitor}, journal = {Nucleic Acids Research}, doi = {10.1093/nar/gkad022}, issn = {0305-1048}, title = {SPRTN-dependent DPC degradation precedes repair of damaged DNA: a proof of concept revealed by the STAR assay}, keyword = {DPC isolation, STAR assay, SPRTN, DNA repair, H2Ax signaling, formaldehyde, topoisomerase inhibitor} }
@article{article, author = {Glumac, Mateo and Polovi\'{c}, Mirjana and Batel, Anja and Gelemanovi\'{c}, Andrea and Ma\v{c}ek, Boris and Veli\'{c}, Ana and Marinovi\'{c}-Terzi\'{c}, Ivana}, year = {2023}, pages = {1-18}, DOI = {10.1093/nar/gkad022}, keywords = {DPC isolation, STAR assay, SPRTN, DNA repair, H2Ax signaling, formaldehyde, topoisomerase inhibitor}, journal = {Nucleic Acids Research}, doi = {10.1093/nar/gkad022}, issn = {0305-1048}, title = {SPRTN-dependent DPC degradation precedes repair of damaged DNA: a proof of concept revealed by the STAR assay}, keyword = {DPC isolation, STAR assay, SPRTN, DNA repair, H2Ax signaling, formaldehyde, topoisomerase inhibitor} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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