Naslov
Immunohistochemical Expression Pattern of Mismatch Repair Genes in the Short-term Streptozotocin- induced Diabetic Rat Kidneys

Autori
Dragun, Matea ; Filipović, Natalija ; Racetin, Anita ; Kostić, Sandra ; Vukojević, Katarina

Izvornik
Applied Immunohistochemistry &amp ; Molecular Morphology (1541-2016) 29 (2021), 9; E83-e91

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
diabetes mellitus ; kidneydiabetic ; nephropathy ; mismatch ; repair genes ; MMR

Sažetak
We studied the expression of mismatch repair genes (MMRs)-mutS protein homolog 2 (MSH2), PMS2, MutL homolog 1 (MLH1), and yH2AFX in diabetic rat kidneys. Streptozotocin-induced diabetes mellitus type 1 rat model (DM1) was used. Renal samples were collected 2 weeks and 2 months after DM1 induction and immunohistochemical expression of MMR genes in the renal cortex was analyzed. Diabetic animals showed lower MSH2 and higher yH2AFX kidney expression both 2 weeks and 2 months after DM1 induction. MLH1 expression significantly increased 2 weeks after DM1 induction (P < 0.0001). The most substantial differences were observed in the period 2 weeks after induction, with lower MSH2 and higher MLH1 expression in the proximal convoluted tubules and distal convoluted tubules (DCT) of diabetic animals (P < 0.001). yH2AFX expression significantly increased in the DCT of diabetic animals at both time points (P < 0.001 ; P < 0.01). PMS2 expression changed only in the glomeruli, where it significantly decreased 2 months after DM1 induction (P < 0.05). We concluded that the most substantial changes in renal expression of MMRs are happening already 2 weeks after diabetes induction, predominantly in the proximal convoluted tubules and DCT. Moreover, DCT could have a critical role in the pathophysiology of diabetic nephropathy (DN) and might be a future therapeutic target in this condition. The obtained results point to the MMRs as a potential factor in the development and progression of DN, as well as the possible link between DN and renal carcinogenesis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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