Naslov
Molecular characterization of the Neisseria meningitidis strains causing invasive disease in Croatia in 2000

Autori
Boras, A ; Jeren, T ; Sacchi, C.T ; Schmink, S ; Popović, T, Members of the Croatian Study Group for Bacterial Meningitis

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3rd Croatian Congress on Infectious Diseases with international participation-abstract book / - Zagreb : Hrvatsko društvo infektologa HLZ-a, 2002

Skup
3rd Croatian Congress on Infectious Diseases with international participation

Mjesto i datum
Dubrovnik, Hrvatska, 12.10.2002. - 15.10.2002

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Neisseria meningitidis

Sažetak
Introduction- The annual incidence of meningococcal disease in Croatia remained stable at 0.7/100, 000 to 1.5/100, 000 over the past ten years. Based on clinical notifications, there were 41 patients with sporadic invasive meningococcal disease in 2000, resulting in the incidence rate of 0.8/100, 000, and mortality of 0.04/100, 000. Twenty-six cases were laboratory confirmed by isolation of N. meningitidis from blood and cerebrospinal fluid. The aim of this study was to molecularly characterize these isolates and assess their relationships to major hypervirulent clonal groups. Materials and Methods- Twenty-three of the 26 isolates were available for molecular characterization by PFGE (NheI) and sequencing of the 16S rRNA gene. Using 10 different primers in forward and reverse orientation, providing 4-7 fold coverage sequenced approximately 1500 bp. Results and Discussion- Serogrouping of the isolates identified 17 (74%) to be serogroup B (MenB), 4 (17%) serogroup C (MenC), one (4%) serogroup W135 (MenW135), while the remaining isolate was nongroupable. Among 17 MenB strains, 10 16S types and 14 PFGE patterns were identified. Only two isolates were of 16S type 4 characteristically seen in strains of the ET-5 complex. Type 25, previously identified in Brazil, Spain, and New Zealand, was identified in three strains. The remaining 12 strains had 8 novel 16S types represented from one to four strains, each. These novel 16S types, as well as 16S type 25 are not characteristic for strains of the ET-5 complex. Excellent correlation was observed between 16S rRNA gene sequencing and PFGE: more than one PFGE pattern was seen in strains with identical 16S type, but no individual PFGE pattern was associated with more than a single 16S type. All four serogroup C strains had decreased susceptibility to penicillin, and were shown to have a novel 16S type 91 ; it differed from 16S type 13, most frequently identified in MenC strains of the ET-37 complex, only by a single base. By PFGE, two very similar patterns were observed, but neither has been previously identified among several hundred serogorup C strains in our database. A single MenW135 isolate represents the first invasive meningococcal disease caused by MenW135 strains in Croatia and is clearly different from the Hajj2000 isolates, by PFGE, 16S typing and porA typing. Conclusion: Molecular characterization of 23 N. meningitidis isolates obtained from patients with sporadic invasive meningococcal disease in Croatia in 2000, demonstrated, revealed substantial level of diversity as shown by PFGE and 16S typing, and indicated that less than one quarter of these strains have molecular markers typical for strains of the ET-5 and ET-37 complex.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA