Pregled bibliografske jedinice broj: 1255839
Spirulina sp. – different drying temperatures and it’s biological activity
Spirulina sp. – different drying temperatures and it’s biological activity // Book of Abstracts of AlgaEurope 2022
Rim, Italija, 2022. str. 225-225 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1255839 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Spirulina sp. – different drying temperatures and
it’s biological activity
Autori
Baković, Marija ; Martić, Ana ; Jurić, Sara ; Punčec, Marina ; Matijević, Gabrijela ; Djedović, Elvis ; Šmuc, Tomislav ; Roje, Marin ; Čož-Rakovac, Rozelindra
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts of AlgaEurope 2022
/ - , 2022, 225-225
Skup
AlgaEurope 2022
Mjesto i datum
Rim, Italija, 13.12.2022. - 15.12.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Spirulina sp. ; Cyanobacteria ; Drying temperature ; Biological activity ; Antioxidant activity
Sažetak
Spirulina sp. is a widely cultivated cyanobacteria which contains a high amount of bioactive compounds with health-promoting effects and remarkable antioxidant activity due to the abundant presence of polyphenols and carotenoids. The effect of different drying temperatures (room temperature, 40, 50, 60, 80, and 100°C) on the phenolic profile and biological activity of Spirulina sp. was investigated. Samples were dried at various temperatures, extracted and then fractioned. Antioxidant activity of methanolic (F3) and dichloromethane (F4) fractions was evaluated using ABTS, FRAP, ORAC and DPPH assays. To determine the toxicity potential of prepared fractions, zebrafish embryotoxicity test was performed. Phenolic compounds in Spirulina sp. samples were identified using HPLC, while LC/ MS-Q-TOF screening was used to predict the most probable bioactivities, chemical classes and drug-likeness properties of examined compounds. According to antioxidative assays, F3 fraction had higher ABTS and FRAP activity, while F4 fraction had higher DPPH and ORAC activity. The F4 fraction dried at 50°C showed the highest antioxidant activity using DPPH (687.06 +/- 11.41 mg AAE/g fraction), while ABTS and FRAP assays showed high activity for F3 fractions dried at 25, 30 and 100°C. There was no negative impact on the survival of zebrafish embryos and larvae observed at concentrations lower than 0.02 mg/mL. The most abundant polyphenols were epicatechin, vanillic acid, caffeic acid, diosmin, thymol, apigenin, while the presence of 3 alkanolamines and 25 lipid-like molecules was revealed by QTOF analysis. High antioxidant activity observed at lower temperatures can be related to the presence of carotenoids and chlorophyll. Antioxidant potential of sample dried at 100°C can be attributed to pigment derivatives. The optimal temperature for preservation of polyphenols was 50°C. A strong correlation between structural class of detected compounds and highly predicted activities (cardiovascular, anti-inflammatory, anticancer) has been observed. The results indicate that drying at a lower temperature retains phenols and other components and maintains high antioxidant activity. It can also be concluded that lower drying temperatures better influence the content of phenolic compounds and biological activity of Spirulina sp., when compared to higher temperatures.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Biotehnologija
POVEZANOST RADA
Projekti:
EK-KF-KK.01.1.1.01.0002 - Bioprospecting Jadranskog mora (Jerković, Igor; Dragović-Uzelac, Verica; Šantek, Božidar; Čož-Rakovac, Rozelinda; Kraljević Pavelić, Sandra; Jokić, Stela, EK ) ( CroRIS)
EK-EFRR-KK.01.2.1.02.0095 - SUPERHRANA – Mikroalgama do inovativnih pekarskih proizvoda i tjestenine (Roje, Marin; Čož-Rakovac, Rozelinda, EK ) ( CroRIS)
EK-EFRR-KK.01.2.1.02.0015 - In silico procjenom bioaktivnosti mikroalgi do razvoja inovativnih biobaziranih proizvoda (Roje, Marin; Čož-Rakovac, Rozelinda, EK ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb
Profili:
Ana Martić
(autor)
Gabrijela Matijević
(autor)
Rozelinda Čož-Rakovac
(autor)
Marija Baković
(autor)
Marin Roje
(autor)
Tomislav Šmuc
(autor)