Naslov
The Influence of Different Drying Temperatures on the Phenolic Profile and Biological Activity of the Cyanobacterium Spirulina sp.

Autori
Baković, Marija ; Jurić, Sara ; Punčec, Marina ; Martić, Ana ; Matijević, Gabrijela ; Djedović, Elvis ; Šmuc, Tomislav ; Roje, Marin ; Čož-Rakovac, Rozelindra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of 15th World Congress on Polyphenols Applications / - , 2022, 80-80

Skup
15th World Congress on Polyphenols Applications

Mjesto i datum
Valencia, Španjolska, 28.09.2022. - 30.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Spirulina sp. ; Drying microalgae ; Biological activity

Sažetak
Spirulina sp. is a widely cultivated microalgae that contains numerous bioactive compounds with health-promoting properties. Phenolic profile and biological activity were investigated in regard to different drying temperatures (25, 40, 50, 60, 80, 100°C). Antioxidant activity (ABTS, FRAP, ORAC, DPPH) of methanolic (F3) and dichloromethane (F4) fractions was evaluated. To assess the toxicity of the fractions, zebrafishembryos were used. Phenolic compounds were identified using HPLC, while prediction of most probable bioactivities, chemical classes, and drug-likeness properties were made using LC/MS-Q-TOF. The F4 (50°C) showed the highest altioxidant activity using DPPH, while ABTS and FRAP showed high activity for F3 (25, 30 and 100°C). No negative impact on the survival of zebrafish was observed at concentrations lower than 0.02 mg/mL. Most abundant polyphenols were epicatechin, vanillic and caffeic acid, while the presence of alkanolamines and lipid-like molecules was revealed by LC/MS-Q-TOF. High antioxidant activity was observed at lower temperatures, due to the presence of carotenoids and chlorophylls, while for the sample dried at 100°C this can be attributed to pigment derivatives. Optimal temperature for preservation of polyphenols was 50°C. A strong correlation between structural class of detected compounds and highly predicted activities (cardiovascular, anti- inflammatory, anticancer) has been observed.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA