Naslov
New cyanine-piperazine derivatives as fluorescent sensors for DNA/RNA secondary structures

Autori
Pšeničnik, Silvia ; Radić Stojković, Marijana ; Crnolatac, Ivo ; Vasilev, Aleksey ; Kandinska, Meglena ; Tumir, Lidija-Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka-Book of Abstracts III. simpozij supramolekulske kemije Supramolecular Chemistry 2019 : knjiga sažetaka = book of abstracts / Frkanec, Leo ; Namjesnik, Danijel ; Tomišić, Vladislav - Zagreb : Institut Ruđer Bošković, 2019, 28-28

ISBN
978-953-7941-27-7

Skup
3. simpozij supramolekulske kemije (Supramolecular Chemistry 2019)

Mjesto i datum
Zagreb, Hrvatska, 03.12.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Piperazine ; Cyanine ; Fluorescence ; Sensor ; DNA ; RNA ; Binding

Sažetak
Design of new sensors and/or biologically active molecules often comprises the combination of different structural subunits that sterically and/or electronically influence on binding to polynucleotides. Cyanine dyes are well known fluorescent probes. They exhibit very small or no intrinsic fluorescence, while their fluorescence emission increases dramatically upon binding to polynucleotides. N-methyl-piperazine group increases binding selectivity of small molecules toward G-quadruplex structures. The newly prepared cyanine-N-methyl- (or N-phenyl)piperazines showed strong binding on different polynucleotides and significant preference on DNA over RNA. Cyanine-N-methyl-piperazines revealed also AT preference over GC sequences. Generally, examined compounds were bound to the polynucleotide minor groove, whereas in case of N-phenyl-piperazine derivatives intercalation into DNA/RNA has been observed. The results of confocal microscopy confirmed the entry of compounds into the cell.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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