Naslov
LONG TERM CENTRAL EFFECTS OF BOTULINUM TOXIN TYPE A ON MUSCULAR FUNCTION AND RECOVERY IN RAT

Autori
Šoštarić, Petra ; Matić, Magdalena ; Matak, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10TH CROATIAN CONGRESS OF PHARMACOLOGY AND THE 1ST CROATIAN CONGRESS OF CLINICAL PHARMACOLOGY WITH INTERNATIONAL PARTICIPATION / Mršić Pelčić, Jasenka ; Vitezić, Dinko ; Janković, Tamara - : Pharmaca, 2022, 132-132

Skup
10th Croatian Congress of Pharmacology ; 1st Croatian Congress of Clinical Pharmacology with International Participation

Mjesto i datum
Opatija, Hrvatska; Zagreb, Hrvatska, 22.09.2022. - 25.09.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Botulinum toxin type A, long term effects

Sažetak
Introduction: Due to presumable action on local neuromuscular terminals, botulinum toxin type A (BoNT-A) is widely used in different muscle hyperactivity disorders. However, clinical observations suggest possible central effects. Herein, we examined the contribution of BoNTA long term central effects on normal muscle function and recovery from paralysis, as well as tetanus neurotoxin (TeNT) evoked spasticity. Materials and methods: BoNT-A was bilaterally injected into rat gastrocnemius muscle (2 U/kg) or sciatic nerve (5 U/kg). To prevent spinal transcytosis, BoNT-A-neutralizing antitoxin was administered into the lumbar intrathecal (i.t.) spine. To examine late antispastic action on disinhibited muscle tone and stretch reflex, 1.5 ng TeNT was injected unilaterally into gastrocnemius muscle on day 62 post BoNT-A. BoNT-A enzymatic activity was examined in injected muscle and spinal cord by cleaved synaptosomal-associated protein 25 (cSNAP-25) immunohistochemistry. Results: I.t. antitoxin significantly accelerated the flaccid paralysis and motor performance recovery in different motor tests (gait ability score, digit abduction score, rota-rod, beam walking and swimming performance). Dependently on its central effects, BoNT-A reduced the TeNT-evoked increased muscle tone, however, did not affect the disinhibited stretch reflex. In contrast to cSNAP-25 continuous presence in injected muscle, BoNT-A action in second order spinal cord cholinergic neurons depended on the toxin’s central transcytosis. Conclusion: Normal motor performance (day 1-62), as well as the spastic paralysis (days 62- 78), are influenced by BoNT-A ongoing central action, suggesting a clinically relevant benefit resulting from combined peripheral and central toxin effects.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
Funding: Croatian Science Foundation (project ID:
UIP-2019-04-8277)

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