Naslov
Methods of determination of progesterone and estradiol in saliva

Autori
Smajli Vokshi, Kaltrina ; Peroš, Kristina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th Croatian Congress of Pharmacology and the 1st Croatian Congress of Clinical Pharmacology with International Participation / Pharmaca 2022 / Mršić-Pelčić, Jasenka ; Vitezić, Dinko ; Janković, Tamara - Zagreb, 2022, 150-150

Skup
10. hrvatski kongres farmakologije ; 1. hrvatski kongres kliničke farmakologije s međunarodnim sudjelovanjem = 10th Croatian Congress of Pharmacology ; 1st Croatian Congress of Clinical Pharmacology and Therapeutics with International Participation

Mjesto i datum
Opatija, Hrvatska, 22.09.2022. - 25.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
progesterone ; estradiol ; saliva

Sažetak
The aim of this study was to analyze methods used to determine the female sex hormones, progesterone and estradiol, in saliva. The literature search was conducted on the PubMed website (US National Library of Medicine, National Institute of Health) in July 2022 using the keywords “female sex hormones, methods of determination, saliva”. The literature search included human studies published in the last 10 years. In this process, 20 published papers were reviewed. According to these articles, saliva samples were stored at -20 °C before analysis, thawed at room temperature, and then centrifuged. The vast majority of articles offer enzyme linked immunosorbent assay (ELISA) kits for the determination of female salivary hormones, although this method is limited to the measurement of only one analyte at a time and smaller samples. In contrast, determination by chromatographic assays has higher sensitivity and multiple hormones can be measured simultaneously. The use of the combined method LC- MS /MS for the measurement of salivary hormones has recently increased, as it has a higher sample throughput and sensitivity and is capable of quantifying small amounts of hormones in saliva. Determination of hormones in saliva has recently become the most attractive method for various medical conditions. Several laboratory methods (ELISA, chromatography, LC-MS /MS) are available for the precise determination of estradiol and progesterone in saliva. The choice of method depends on the study design and the characteristics of the saliva sample.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Dentalna medicina

POVEZANOST RADA