Naslov
Gut microbiota disparities between juvenile idiopathic and reactive arthritis patients at the initial stage of the disease

Autori
Radoš, Ivana ; Vidović, Mandica ; Cindrić, Mario ; Hozić, Amela ; Buljan, Domagoj ; Paleka Bosak, Edi ; Harjaček, Miroslav ; Lamot, Lovro

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Annual meeting of the Croatian Immunological Society 2019 : Abstract Book / - , 2019, 27-27

Skup
Annual meeting of the Croatian Immunological Society 2019

Mjesto i datum
Rovinj, Hrvatska, 11.10.2019. - 12.10.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
gut microbiota ; juvenile idiopathic arthritis ; reactive arthritis ; E. coli ; pediatrics

Sažetak
INTRODUCTION: Painful joint swelling is a symptom of many childhood diseases, most notably reactive arthritis (ReA) and juvenile idiopathic arthritis (JIA). The growing body of evidence suggests that gut microbiota could orchestrate the immune response and development of those diseases. This study aimed to assess the differences in the presence of Escherichia coli (E coli) subtypes in the stool of JIA and ReA patients at the first occurrence of symptoms. METHODS: Stool samples of 14 patients with joint swelling were collected during their first visit to Pediatric Rheumatology Clinic in Sestre milosrdnice University Hospital Center in Zagreb, Croatia. The samples were analyzed by mass spectrometry on nanoLC-Synapt G2 Si instrument. To identify the most abundant E coli subtypes, specialized software named Protein Reader with implemented Dust algorithm searched through NCBI nr database. RESULTS: Various E coli subtypes (P0301867.1-10, O104:H4, O103:H25, O111:H11, KTE and K) were three times more abundant in patients with JIA, while increased abundance of diarrheagenic E. coli (DEC) was detected in children with ReA. CONCLUSION: This pilot study has shown differences in the subtypes of E. coli present in the stool of children with ReA and JIA in the early stage of the disease. Since E coli is one of the paramount bacteria in gut microbiota, it is reasonable to assume that the differences described in this study can have a potential impact on the gut environment, contributing to the development of the chronic disease in JIA patients, or the resolution of symptoms in children with ReA.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA