Naslov
Influence of phenanthridinium-nucleobase conjugates protonation / methylation on their interactions with polynucleotides

Autori
Piantanida, Ivo ; Tumir, L.-M. ; Žinić, Mladen ; Juranović Cindrić, Iva ; Meić, Zlatko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XVIII. Hrvatski skup kemičara i kemijskih inženjera / Zrnčević, S. - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Hrvatsko kemijsko društvo, 2003, 18-18

Skup
XVIII. Hrvatski skup kemičara i kemijskih inženjera

Mjesto i datum
Zagreb, Hrvatska, 16.02.2003. - 19.02.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
phenanthridinium - nucleobase conjugate; polynucleotides; protonation

Sažetak
Prijepis sažetka na izvornom jeziku Interactions of different intercalator - nucleobase conjugates with targeted polynucleotides were an objective of intensive research in last decade. A number of conjugates were found to exhibit selective binding at complementary abasic sites in double stranded DNA / RNA [1]. Some of them also showed specific scission of double strand [1]. However, there was no systematic study on influence of the type of intercalator charge on the binding properties of conjugates. Therefore, we have studied interactions of a number of novel phenanthridinium - nucleobase conjugates differing in a protonated / methylated aromatic nitrogen of phenanthridinium unit with RNA [2-4]. Adenine conjugate of structure B (protonated N) has shown higher affinity toward complementary poly U compared with reference and uracil derivative [3] while respective conjugate of structure A (methylated N) under same (acidic) conditions binds preferentially to ds- poly AH+ and strongly stabilises its double helix [4]. The results clearly demonstrate the importance of a type of charge in the intercalator moiety. Taking this parameter in account is necessary for successful design of small molecules with recognition of nucleobases of single stranded polynucleotide targets.[1] Lhomme J. et al, Biochemistry 38 (1999) 1930-7 and ref. cited therein. ; [2] Tumir L.-M., Piantanida I., Novak P., Žinić M., J. Phys. Org. Chem. 15 (2002) 599-607. ; [3] Juranović I., Meić Z., Piantanida I., Tumir L.-M., Žinić M., Chem. Commun. (2002) 1432-3. ; [4] Tumir L.-M., Piantanida I., Žinić M., Juranović I., Meić Z., submitted[5] a) Scatchard G., Ann. N.Y. Acad. Sci., 51 (1949) 660-664 ; b) McGhee J.D., von Hippel P.H., J. Mol. Biol. 86 (1974) 469-489.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA