Naslov
Utjecaj eluiranih monomera dentalnih kompozita na embrionalni razvoj modelnog organizma zebrice Danio rerio
(Influence of monomers eluted from dental composites on embryonic development of Danio rerio zebrafish model organism)

Autori
Majstorović Matejić, Matea ; Marović, Danijela ; Malev, Olga ; Par, Matej ; Tarle, Zrinka ; Čož-Rakovac, Rozelindra ; Babić, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8. Međunarodni kongres Stomatološkog fakulteta Sveučilišta u Zagrebu. U: Acta Stomatologica Croatica. 2022 ; 56(2) / Klarić Sever, Eva - Zagreb, 2022, 197-198

Skup
8. Međunarodni kongres Stomatološkog fakulteta Sveučilišta u Zagrebu

Mjesto i datum
Rovinj, Hrvatska, 08.04.2022. - 09.04.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
zebrice ; embriotoksičnost ; kompozit ; eluati ; HPLC
(zebrafish ; embryotoxicity ; resin composite ; eluates ; HPLC)

Sažetak
Aim: After removing dental restorative materials, their dust goes into the wastewater, and enters the aquatic ecosystems. Previous studies based on the study of (eco)toxic potential of dental materials were mostly based on cell lines while neglecting the effects on model vertebrate organisms. For this reason, this research aims to determine the influence of microparticles of composite materials and their eluates on the embryonic development of the zebrafish Danio rerio. Materials and methods: This study included two types of composite materials: commercial composite (Tetric EvoCeram, Ivoclar Vivadent) and laboratory composite (30% BisGMA/TEGDMA 60:40, 70% filler). Composite samples (d=6mm, h=2mm) were polymerized with Bluephase G2 (Ivoclar Vivadent ; 1200mW/cm2) for 10s. The samples were then ground, their powder collected, and dispersed in zebrafish medium. Samples were incubated on a shaker for 48h and 7 days, after which an embryotoxicity test was performed on Danio rerio embryos. In addition to mortality rates and morphological abnormalities, the cardiotoxic and neurotoxic potential was monitored. The chemical composition of the eluates was determined by high-performance liquid chromatography (Agilent 1260 Bioinert) to identify released monomers. Results: Only UDMA at a concentration of 1.21mg/ml was detected in the 48h-eluates of Tetric EvoCeram, which was present at a reduced concentration of 0.69mg/mL in 7d- eluates. TEGDMA (0.016mg/mL) was detected in the laboratory composite after 48h, with the same concentration after 7 days. Composite microparticles showed a negative effect on the embryonic development of zebrafish depending on the tested concentration. The most commonly observed developmental abnormalities during exposure were pericardial edema and scoliosis. Both tested composites caused increased heart activity. The reduction of the individuals length, the lack of pigmentation, and the accelerated pectoral fin movement were more pronounced in the commercial composite. Conclusion: The embryotoxicity of the tested eluates of dental composites was largest after 48h of incubation in the medium. The obtained results highlight the potentially negative impact on aquatic ecosystems and emphasize the importance of timely removal and disposal of these materials.

Izvorni jezik
Hrvatski, engleski

Znanstvena područja
Kemija, Biologija, Dentalna medicina, Interdisciplinarne biotehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA