Naslov
Interactions of phenanthridinium-nucleobase conjugates with complementary and non-complementary nucleotides in aqueous media

Autori
Juranović Cindrić, Iva ; Meić, Zlatko ; Piantanida, Ivo ; Tumir, L.-M. ; Žinić, Mladen

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XVIII. hrvatski skup kemičara i kemijskih inženjera : sažeci = abstracts / Zrnčević, Stanka - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo ; Hinus, 2003, 9-9

ISBN
9536894084

Skup
Hrvatski skup kemičara i kemijskih inženjera (18 ; 2003)

Mjesto i datum
Zagreb, Hrvatska, 16.02.2003. - 19.02.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
phenanthridinium – nucleobase conjugate; polynucleotides; fluorimetric titration

Sažetak
Interactions of a number of intercalator – nucleobase conjugates with targeted nucleotides were recently intensively studied [ ]. Among the others, some phenanthridinium – nucleobase conjugates have shown preferential binding toward complementary polynucleotides [ ]. In order to study possible additivity and consequently to achieve a higher selectivity we have prepared novel conjugates with the nucleobases linked to both sides of the phenanthridinium unit by a C3 methylene chain. Addition of nucleotides to aqueous solution of phenanthridinium-nucleobase conjugates 1-3 showed significant changes in their fluorescence emission and UV/Vis spectra. Fluorimetric titrations indicate that phenanthridinium-nucleobase conjugates 1-3 form non-covalent complexes with nucleotides in aqueous solutions. The stoichiometry of the complexes was 1:1 and binding constants were in the range 102 – 101 dm3 mol-1. No significant preferential binding was observed for conjugates with complementary nucleotides (2 and 3). Aromatic p-p stacking interactions are found to be dominant in complexes of all studied conjugates with nucleotides

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA