Naslov
Structural investigation of waste biomass-derived carbon for bioelectrocatalytic applications

Autori
Rešetar, Egon ; Plodinec, Milivoj ; Kurajica, Stanislav ; Iveković, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th Multinational Congress on Microscopy - Book of Abstracts / Gajović, Andreja ; Weber, Igor ; Kovačević, Goran ; Čadež, Vida ; Šegota, Suzana ; Peharec Štefanić, Petra ; Vidoš, Ana - Zagreb : Institut Ruđer Bošković ; Hrvatsko mikroskopijsko društvo, 2017, 683-684

ISBN
978-953-7941-19-2

Skup
13th Multinational Congress on Microscopy

Mjesto i datum
Rovinj, Hrvatska, 24.09.2017. - 29.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Pyrolytic carbon, bioelectrocatalysis, direct electron transfer, HRTEM, STEMHAADF

Sažetak
Biomass-derived carbon (BDC) is environmentally friendly and cost competitive material produced by pyrolysis of plant (lignocellulosic) biomass. Due to its electric conductivity combined with high porosity and tunable surface properties, BDC has recently attracted considerable interest in the field of electrochemistry, primarily as an electrode material for electrochemical supercapacitors and high-performance metal-ion batteries or as an electrocatalyst for oxygen reduction and hydrogen evolution reactions (1, 2). In this work, we studied the suitability of BDC as a substrate for enzyme immobilization and its applicability as electrode material in bioelectrocatalysis. Conductive carbon samples were prepared by rapid pyrolysis (at a heating rate of 100 °C/min) of spent coffee grounds in the temperature range between 600 and 900 °C in an inert (N2) atmosphere, with the addition of iron salt as a graphitization catalyst. The obtained pyrolytic carbon was employed as a substrate for immobilization of horseradish peroxidase (HRP), a model redox enzyme that catalyzes the reduction of hydrogen peroxide in the presence of a suitable organic electron donor or, in the special case of HRP adsorbed on electrode, through the process of direct electron transfer between the enzyme redox-center and conductive substrate, usually referred as a 'bioelectrocatalysis'. It was observed that both the bioelectrocatalytic activity and the stability of immobilized enzyme were significantly better for HRP immobilized on pyrolytic carbon samples prepared in this work, than for HRP immobilized on graphite, a commonly employed electrode material in bioelectrocatalysis. Observed enhancement of the bioelectrocatalytic properties of HRP will be discussed in terms of the structural characteristics of prepared pyrolytic carbon samples (porosity, phase composition, density of surface groups, relative abundance of sp2- and sp3-hybridized domains, disorder and deformation of the carbon layers within the graphitic domains), as determined by scanning electron microscopy (SEM), X-ray diffraction (XRD), infrared and Raman spectroscopy, high-resolution transmission electron microscopy (HRTEM), and high angle annular dark field scanning transmission electron microscopy (STEM-HAADF).

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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