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Pregled bibliografske jedinice broj: 1250396

Measuring DNA modifications with the comet assay: a compendium of protocols


(Cost Action CA 15132) Collins, Andrew; Møller, Peter; Gajski, Goran; Vodenková, Soňa; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, Stefano; Boutet-Robinet, Elisa; Brunborg, Gunnar et al.
Measuring DNA modifications with the comet assay: a compendium of protocols // Nature Protocols, 1 (2023), 1038; 1038, 61 doi:10.1038/s41596-022-00754-y (međunarodna recenzija, pregledni rad, znanstveni)


CROSBI ID: 1250396 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Measuring DNA modifications with the comet assay: a compendium of protocols

Autori
Collins, Andrew ; Møller, Peter ; Gajski, Goran ; Vodenková, Soňa ; Abdulwahed, Abdulhadi ; Anderson, Diana ; Bankoglu, Ezgi Eyluel ; Bonassi, Stefano ; Boutet-Robinet, Elisa ; Brunborg, Gunnar ; Chao, Christy ; Cooke, Marcus S. ; Costa, Carla ; Costa, Solange ; Dhawan, Alok ; de Lapuente, Joaquin ; Bo’, Cristian Del ; Dubus, Julien ; Dusinska, Maria ; Duthie, Susan J. ; Yamani, Naouale El ; Engelward, Bevin ; Gaivão, Isabel ; Giovannelli, Lisa ; Godschalk, Roger ; Guilherme, Sofia ; Gutzkow, Kristine B. ; Habas, Khaled ; Hernández, Alba ; Herrero, Oscar ; Isidori, Marina ; Jha, Awadhesh N. ; Knasmüller, Siegfried ; Kooter, Ingeborg M. ; Koppen, Gudrun ; Kruszewski, Marcin ; Ladeira, Carina ; Laffon, Blanca ; Larramendy, Marcelo ; Hégarat, Ludovic Le ; Lewies, Angélique ; Lewinska, Anna ; Liwszyc, Guillermo E. ; de Cerain, Adela López ; Manjanatha, Mugimane ; Marcos, Ricard ; Milić, Mirta ; de Andrade, Vanessa Moraes ; Moretti, Massimo ; Muruzabal, Damian ; Novak, Matjaž ; Oliveira, Rui ; Olsen, Ann-Karin ; Owiti, Norah ; Pacheco, Mário ; Pandey, Alok K. ; Pfuhler, Stefan ; Pourrut, Bertrand ; Reisinger, Kerstin ; Rojas, Emilio ; Rundén-Pran, Elise ; Sanz-Serrano, Julen ; Shaposhnikov, Sergey ; Sipinen, Ville ; Smeets, Karen ; Stopper, Helga ; Teixeira, João Paulo ; Valdiglesias, Vanessa ; Valverde, Mahara ; van Acker, Frederique ; van Schooten, Frederik-Jan ; Vasquez, Marie ; Wentzel, Johannes F. ; Wnuk, Maciej ; Wouters, Annelies ; Žegura, Bojana ; Zikmund, Tomas ; Langie, Sabine A. S. ; Azqueta, Amaya

Kolaboracija
Cost Action CA 15132

Izvornik
Nature Protocols (1754-2189) 1 (2023), 1038; 1038, 61

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
alkaline comet assay ; protocols

Sažetak
The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Farmacija, Interdisciplinarne biotehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb

Profili:

Avatar Url Mirta Milić (autor)

Avatar Url Goran Gajski (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

(Cost Action CA 15132) Collins, Andrew; Møller, Peter; Gajski, Goran; Vodenková, Soňa; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, Stefano; Boutet-Robinet, Elisa; Brunborg, Gunnar et al.
Measuring DNA modifications with the comet assay: a compendium of protocols // Nature Protocols, 1 (2023), 1038; 1038, 61 doi:10.1038/s41596-022-00754-y (međunarodna recenzija, pregledni rad, znanstveni)
(Cost Action CA 15132) (Cost Action CA 15132) Collins, A., Møller, P., Gajski, G., Vodenková, S., Abdulwahed, A., Anderson, D., Bankoglu, E., Bonassi, S., Boutet-Robinet, E. & Brunborg, G. (2023) Measuring DNA modifications with the comet assay: a compendium of protocols. Nature Protocols, 1 (1038), 1038, 61 doi:10.1038/s41596-022-00754-y.
@article{article, author = {Collins, Andrew and M\oller, Peter and Gajski, Goran and Vodenkov\'{a}, So\v{n}a and Abdulwahed, Abdulhadi and Anderson, Diana and Bankoglu, Ezgi Eyluel and Bonassi, Stefano and Boutet-Robinet, Elisa and Brunborg, Gunnar and Chao, Christy and Cooke, Marcus S. and Costa, Carla and Costa, Solange and Dhawan, Alok and de Lapuente, Joaquin and Bo’, Cristian Del and Dubus, Julien and Dusinska, Maria and Duthie, Susan J. and Yamani, Naouale El and Engelward, Bevin and Gaiv\~{a}o, Isabel and Giovannelli, Lisa and Godschalk, Roger and Guilherme, Sofia and Gutzkow, Kristine B. and Habas, Khaled and Hern\'{a}ndez, Alba and Herrero, Oscar and Isidori, Marina and Jha, Awadhesh N. and Knasm\"{u}ller, Siegfried and Kooter, Ingeborg M. and Koppen, Gudrun and Kruszewski, Marcin and Ladeira, Carina and Laffon, Blanca and Larramendy, Marcelo and H\'{e}garat, Ludovic Le and Lewies, Ang\'{e}lique and Lewinska, Anna and Liwszyc, Guillermo E. and de Cerain, Adela L\'{o}pez and Manjanatha, Mugimane and Marcos, Ricard and Mili\'{c}, Mirta and de Andrade, Vanessa Moraes and Moretti, Massimo and Muruzabal, Damian and Novak, Matja\v{z} and Oliveira, Rui and Olsen, Ann-Karin and Owiti, Norah and Pacheco, M\'{a}rio and Pandey, Alok K. and Pfuhler, Stefan and Pourrut, Bertrand and Reisinger, Kerstin and Rojas, Emilio and Rund\'{e}n-Pran, Elise and Sanz-Serrano, Julen and Shaposhnikov, Sergey and Sipinen, Ville and Smeets, Karen and Stopper, Helga and Teixeira, Jo\~{a}o Paulo and Valdiglesias, Vanessa and Valverde, Mahara and van Acker, Frederique and van Schooten, Frederik-Jan and Vasquez, Marie and Wentzel, Johannes F. and Wnuk, Maciej and Wouters, Annelies and \v{Z}egura, Bojana and Zikmund, Tomas and Langie, Sabine A. S. and Azqueta, Amaya}, year = {2023}, pages = {61}, DOI = {10.1038/s41596-022-00754-y}, chapter = {1038}, keywords = {alkaline comet assay, protocols}, journal = {Nature Protocols}, doi = {10.1038/s41596-022-00754-y}, volume = {1}, number = {1038}, issn = {1754-2189}, title = {Measuring DNA modifications with the comet assay: a compendium of protocols}, keyword = {alkaline comet assay, protocols}, chapternumber = {1038} }
@article{article, author = {Collins, Andrew and M\oller, Peter and Gajski, Goran and Vodenkov\'{a}, So\v{n}a and Abdulwahed, Abdulhadi and Anderson, Diana and Bankoglu, Ezgi Eyluel and Bonassi, Stefano and Boutet-Robinet, Elisa and Brunborg, Gunnar and Chao, Christy and Cooke, Marcus S. and Costa, Carla and Costa, Solange and Dhawan, Alok and de Lapuente, Joaquin and Bo’, Cristian Del and Dubus, Julien and Dusinska, Maria and Duthie, Susan J. and Yamani, Naouale El and Engelward, Bevin and Gaiv\~{a}o, Isabel and Giovannelli, Lisa and Godschalk, Roger and Guilherme, Sofia and Gutzkow, Kristine B. and Habas, Khaled and Hern\'{a}ndez, Alba and Herrero, Oscar and Isidori, Marina and Jha, Awadhesh N. and Knasm\"{u}ller, Siegfried and Kooter, Ingeborg M. and Koppen, Gudrun and Kruszewski, Marcin and Ladeira, Carina and Laffon, Blanca and Larramendy, Marcelo and H\'{e}garat, Ludovic Le and Lewies, Ang\'{e}lique and Lewinska, Anna and Liwszyc, Guillermo E. and de Cerain, Adela L\'{o}pez and Manjanatha, Mugimane and Marcos, Ricard and Mili\'{c}, Mirta and de Andrade, Vanessa Moraes and Moretti, Massimo and Muruzabal, Damian and Novak, Matja\v{z} and Oliveira, Rui and Olsen, Ann-Karin and Owiti, Norah and Pacheco, M\'{a}rio and Pandey, Alok K. and Pfuhler, Stefan and Pourrut, Bertrand and Reisinger, Kerstin and Rojas, Emilio and Rund\'{e}n-Pran, Elise and Sanz-Serrano, Julen and Shaposhnikov, Sergey and Sipinen, Ville and Smeets, Karen and Stopper, Helga and Teixeira, Jo\~{a}o Paulo and Valdiglesias, Vanessa and Valverde, Mahara and van Acker, Frederique and van Schooten, Frederik-Jan and Vasquez, Marie and Wentzel, Johannes F. and Wnuk, Maciej and Wouters, Annelies and \v{Z}egura, Bojana and Zikmund, Tomas and Langie, Sabine A. S. and Azqueta, Amaya}, year = {2023}, pages = {61}, DOI = {10.1038/s41596-022-00754-y}, chapter = {1038}, keywords = {alkaline comet assay, protocols}, journal = {Nature Protocols}, doi = {10.1038/s41596-022-00754-y}, volume = {1}, number = {1038}, issn = {1754-2189}, title = {Measuring DNA modifications with the comet assay: a compendium of protocols}, keyword = {alkaline comet assay, protocols}, chapternumber = {1038} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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