Naslov
Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid

Autori
Berni, Francesca ; Kalfopoulou, Ermioni ; Gimeno Cardells, Ana M. ; Carboni, Filippo ; van der Es, Daan ; Romero-Saavedra, Felipe ; Laverde, Diana ; Miklic, Karmela ; Malic, Suzana ; Rovis, Tihana L. ; Jonjic, Stipan ; Ali, Sara ; Overkleeft, Herman S. ; Hokke, Cornelis H. ; van Diepen, Angela ; Adamo, Roberto ; Jiménez-Barbero, Jesús ; van der Marel, Gijsbert A. ; Huebner, Johannes ; Codée, Jeroen D. C.

Izvornik
ACS Chemical Biology (1554-8929) 16 (2021), 8; 1344-1349

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pismo, znanstveni

Ključne riječi
Teichoic Acids ; Staphylococcus ; Enterococcus ; Sugars ; Antibodies, Monoclonal ; Phosphates ; Surface Plasmon Resonance ; Enzyme-Linked Immunosorbent Assay ; Magnetic Resonance Spectroscopy ; Binding Sites

Sažetak
Glycerol phosphate (GroP)-based teichoic acids (TAs) are antigenic cell-wall components found in both enterococcus and staphylococcus species. Their immunogenicity has been explored using both native and synthetic structures, but no details have yet been reported on the structural basis of their interaction with antibodies. This work represents the first case study in which a monoclonal antibody, generated against a synthetic TA, was developed and employed for molecular-level binding analysis using TA microarrays, ELISA, SPR- analyses, and STD-NMR spectroscopy. Our findings show that the number and the chirality of the GroP residues are crucial for interaction and that the sugar appendage contributes to the presentation of the backbone to the binding site of the antibody

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA