Naslov
The Virus-Induced Upregulation of the miR- 183/96/182 Cluster and the FoxO Family Protein Members Are Not Required for Efficient Replication of HSV-1

Autori
Andreja Zubković, Ines Žarak, Ivana Ratkaj, Filip Rokić, Maja Jekić, Marina Pribanić Matešić, Ricardo Lebrón, Cristina Gómez-Martín, Berislav Lisnić, Vanda Juranić Lisnić, Stipan Jonjić, Dongli Pan, Oliver Vugrek, Michael Hackenberg, Igor Jurak

Izvornik
Viruses (1999-4915) 14 (2022), 1661; 1-18

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
herpes simplex virus 1 ; HSV-1 ; virus-host interaction ; miRNA ; FoxO

Sažetak
Abstract: Herpes simplex virus 1 (HSV-1) expresses a large number of miRNAs, and their function is still not completely understood. In addition, HSV- 1 has been found to deregulate host miRNAs, which adds to the complexity of the regulation of efficient virus replication. In this study, we comprehensively addressed the deregulation of host miRNAs by massive-parallel sequencing. We found that only miRNAs expressed from a single cluster, miR- 183/96/182, are reproducibly deregulated during productive infection. These miRNAs are predicted to regulate a great number of potential targets involved in different cellular processes and have only 33 shared targets. Among these, members of the FoxO family of proteins were identified as potential targets for all three miRNAs. However, our study shows that the upregulated miRNAs do not affect the expression of FoxO proteins, moreover, these proteins were upregulated in HSV-1 infection. Furthermore, we show that the individual FoxO proteins are not required for efficient HSV-1 replication. Taken together, our results indicate a complex and redundant response of infected cells to the virus infection that is efficiently inhibited by the virus.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

Napomena
This research was funded by the Croatian Science
Foundation (grants #8790 and #2287), MC-CIG
#618587; ESF HR.3.2.01-0330; and University of
Rijeka support grant to I.J.; and the European
Regional Development Fund (“Strengthening the
capacity of the Scientific Center of Excellence
CerVirVac for research in viral immunology and
vaccinology”; KK.01.1.1.01.0006).

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