Pregled bibliografske jedinice broj: 1246277
Green Solid-Phase (Micro)Extraction of Andrographolides’ from Human Plasma Samples Followed by UHPLC-DAD-QqQ-MS/MS Analysis
Green Solid-Phase (Micro)Extraction of Andrographolides’ from Human Plasma Samples Followed by UHPLC-DAD-QqQ-MS/MS Analysis // Separations, 10 (2023), 2; 69-87 doi:10.3390/separations10020069 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1246277 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Green Solid-Phase (Micro)Extraction of
Andrographolides’
from Human Plasma Samples Followed by
UHPLC-DAD-QqQ-MS/MS Analysis
Autori
Kovačić, Jelena ; Jeličić, Mario-Livio ; Amidžić Klarić, Daniela ; Mornar, Ana
Izvornik
Separations (2297-8739) 10
(2023), 2;
69-87
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
andrographolides ; bioanalysis ; sample preparation ; MEPS ; green analytical chemistry
Sažetak
Since inflammatory bowel disease is a progressive disease, optimal treatment early in the disease course is paramount. Still, the use of conventional drugs is accompanied by side effects. The herbal therapies for gastrointestinal disorders are traditionally widespread in Asia, while in Western countries have been adopted in recent years. Andrographis paniculata is a herbaceous plant used in antiinflammatory treatment. To improve its clinical application, an analytical method with the potential to define a relationship between dose and plasma concentration of active ingredients is prerequisite. Considering the increasing demand for eco-friendly bioanalytical methods, our research aimed to implement new sample preparation technique microextraction by packed sorbent for extraction of marker compounds of A. paniculata from plasma. This green procedure using polymeric sorbent based on the poly (styrene-divinylbenzene) allowed miniaturization of sample volume (100 µL), decrease in consumption of solvents, and high recovery (92–98%). A highly selective and sensitive reversed-phase ultrahigh-performance liquid chromatography method has been developed and validated. The newest greenness assessment software AGREE calculator was used for evaluation of the method’s environmental performance. It suggests that our method conforms to the eco- friendly conditions (score 0.8). The sample preparation was identified as weak point of the bioanalytical methods which highlights the importance of microextraction.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Farmacija
POVEZANOST RADA
Projekti:
UIP-2017-05-3949 - Razvoj naprednih analitičkih metoda za lijekove i biološki aktivne tvari u liječenju upalnih bolesti crijeva (IBDAnalytics) (Mornar Turk, Ana, HRZZ - 2017-05) ( CroRIS)
EK-EFRR-KK.01.1.1.02.0021 - Jačanje znanstveno-istraživačkih i inovacijskih kapaciteta Farmaceutsko-biokemijskog fakulteta Sveučilišta u Zagrebu (FarmInova) (Lovrić, Jasmina, EK - Ulaganje u organizacijsku reformu i infrastrukturu u sektoru istraživanja, razvoja i inovacija) ( CroRIS)
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb
Profili:
Ana Mornar Turk
(autor)
Daniela Amidžić Klarić
(autor)
Mario-Livio Jeličić
(autor)
Jelena Kovačić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus