Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes

Konjević, Dean; Erman, Vlatka; Bujanić, Miljenko; Svetličić, Ida; Arbanasić, Haidi; Lubura Strunjak, Snježana; Galov, Ana

doi:10.3390/pathogens11111359

Pregled bibliografske jedinice broj: 1245761

Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes

Konjević, Dean; Erman, Vlatka; Bujanić, Miljenko; Svetličić, Ida; Arbanasić, Haidi; Lubura Strunjak, Snježana; Galov, Ana

Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes // Pathogens, 11 (2022), 1359, 9 doi:10.3390/pathogens11111359 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1245761 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes

Autori
Konjević, Dean ; Erman, Vlatka ; Bujanić, Miljenko ; Svetličić, Ida ; Arbanasić, Haidi ; Lubura Strunjak, Snježana ; Galov, Ana

Izvornik
Pathogens (2076-0817) 11 (2022); 1359, 9

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
fascioloidosis ; swine leucocyte antigens ; host–parasite interactions

Sažetak
Fascioloidosis is a parasitic disease caused by a trematode Fascioloides magna. Since major histocompatibility complex (MHC) genes play an important role in the immune response, the aim of this study was to compare the potential differences in MHC class II SLA-DRB1 exon 2 genes between wild boar populations from infected (cases) and non-infected areas (controls). During the winter of 2021, a total of 136 wild boar tissue samples were collected, 39 cases and 97 controls. DNA was extracted and sequenced using the Illumina platform. Differences in distributions of allele combinations were calculated using the Chi-Square test for homogeneity and between proportions using the large-sample test and Fisher–Irwin test. Analysis revealed 19 previously described swine leucocyte antigen (SLA) alleles. The number of polymorphic sites was 79 (29.6%), with 99 mutations in total. Nucleotide diversity π was estimated at 0.11. Proportions of the alleles SLA-DRB1*12:05 (p = 0.0008379) and SLA-DRB1*0101 (p = 0.0002825) were statistically significantly higher in controls, and proportions of the SLA-DRB1*0602 (p = 0.006059) and SLA-DRB1*0901 (p = 0.0006601) in cases. Alleles SLA-DRB1*04:09, SLA-DRB1*0501, SLA- DRB1*11:09, and SLA-DRB1*1301 were detected only in cases, while SLA-DRB1*0404, SLA-DRB1*0701, SLA- DRB1*02:10, and SLA-DRB1*04:08 were present only in controls. We did not confirm the existence of specific alleles that could be linked to F. magna infection. Detected high variability of the MHC class II SLA-DRB1 exon 2 genes indicate high resistance potential against various pathogens.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina

POVEZANOST RADA

Projekti:
HRZZ-IP-2018-01-8963 - Interakcija nositelj-parazit: odnos tri različita tipa nositelja prema invaziji metiljem Fascioloides magna (MAGNA) (Konjević, Dean, HRZZ - 2018-01) ( CroRIS)

Ustanove:
Veterinarski fakultet, Zagreb

Profili:

Ana Galov (autor)