Naslov
Dose dependent effects of standardized nose-horned viper (Vipera ammodytes ammodytes) venom on parameters of cardiac function in isolated rat heart.

Autori
Lukšić, Boris ; Brizić, Ivica ; Lang Balija M ; Modun, Darko ; Čulić, Viktor ; Halassy, B ; Salamunić, I ; Boban, Mladen

Izvornik
Comparative biochemistry and physiology. C. Toxicology & pharmacology (1532-0456) 147 (2008), 4; 434-440

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Coronary Circulation / drug effects ; Coronary Circulation / physiology ; Rats ; Troponin I / metabolism ; Ventricular Pressure / drug effects ; Ventricular Pressure / physiology ; Viper Venoms / toxicity ; Viperidae

Sažetak
Direct, dose dependent effects of the nose-horned vipers (Vipera ammodytes ammodytes) venom on various parameters of cardiac action in isolated rat hearts were examined. Biochemical (protein content, SDS polyacrylamide gel electrophoresis) and biological (minimum haemorrhagic and necrotizing dose and lethal dose (LD(50))) characterization of the venom was performed before testing. The hearts were infused with venom doses of 30, 90 and 150 microg/mL for 10 min followed by 30 min of wash out period. Left ventricular pressure, coronary flow, heart rate, atrioventricular conduction, myocardial oxygen consumption, incidence and duration of arrhythmias were measured and relative cardiac efficiency was calculated. Cardiac CPK, LDH, AST and troponin I were measured as biochemical markers of myocardial damage. The venom caused dose dependent electrophysiological instability and depression of contractility and coronary flow. Effects on the heart rate were biphasic ; transient increase followed by significant slowing of the frequency. Relative cardiac efficiency decreased as oxygen consumption remained high relative to the heart rate-contractility product, indicating purposeless expenditure of oxygen and energy. Effects by the dose of 30 microg/mL were highly reversible while the dose of 90 mug/mL caused damages that were mostly irreversible. The dose of 150 mug/mL induced irreversible asystolic cardiac arrest.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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