Pregled bibliografske jedinice broj: 1244424
Biocatalyst improvement of halohydrin dehalogenase
Biocatalyst improvement of halohydrin dehalogenase // Slovenski kemijski dnevi 2022 = 28th Annual Meeting of the Slovenian Chemical Society : zbornik povzetkov = book of abstracts
Portorož, Slovenija, 2022. str. - (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1244424 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Biocatalyst improvement of halohydrin dehalogenase
Autori
Vasić, Katja ; Milčić, Nevena ; Findrik Blažević, Zvjezdana ; Majerić Elenkov, Maja ; Knez, Željko ; Leitgeb, Maja
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Slovenski kemijski dnevi 2022 = 28th Annual Meeting of the Slovenian Chemical Society : zbornik povzetkov = book of abstracts
/ - , 2022
Skup
SCS Annual Meeting 2022
Mjesto i datum
Portorož, Slovenija, 21.09.2022. - 23.09.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
halohydrin dehalogenase ; magnetic nanoparticles ; functionalization ; immobilization ; cross-linking
Sažetak
Halohydrin dehalogenases (HHDHs) are important and versatile biocatalysts, that can be used for the synthesis of important building blocks and precursors in the pharmaceutical, agrochemical and chemical industries. HHDHs have a wide spectrum of different compounds, such as chiral epoxides, β-substituted alcohols, oxazolidinones and others, that can be synthesized by their action. However, the enzyme was reported to lack stability under operational conditions. Therefore, immobilization of HHDH onto magnetic nanoparticles (MNPs) represents a powerful tool to overcome its limitations and improve its enzyme characteristics as a biocatalyst. HHDH was immobilized onto bare maghemite nanoparticles, chitosan coated MNPs and carboxymethyl dextran (CMD) coated MNPs. All types of MNPs were prepared by co-precipitation method of ferrous and ferric ions. The immobilization protocol was initially optimized using 10% (v/v) cross-linker glutaraldehyde (GA) in order to improve residual activity and immobilization efficiency of immobilized HHDH. The highest immobilization efficiency (91%) of immobilized HHDH was achieved, while using CMD coated MNPs, which were cross-linked with 10% (v/v) GA for 1 hour at 350 rpm and later immobilized with HHDH for 1 hour at 350 rpm. To implement HHDH at industrial scale, initial optimization of HHDH immobilization was performed, which offers room for improvement in order to achieve an effective biocatalyst for various synthetic applications.
Izvorni jezik
Engleski
Znanstvena područja
Kemijsko inženjerstvo