Naslov
Structural Bases for Selectivity of Butyrylcholinesterase towards Enantiomeric Quinuclidin-3-yl Benzoates: a Quantum Chemical Study

Autori
Primožič, Ines ; Hrenar, Tomica ; Tomić, Srđanka ; Meić, Zlatko

Izvornik
Croatica Chemica Acta (0011-1643) 76 (2003); 93-99

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
butyrylcholinesterase; benzoylcholine; (R)- and (S)-quinuclidin-3-yl benzoates; semiempirical calculations; Michaelis complexes; tetrahedral intermediates; mechanism of hydrolysis

Sažetak
In order to explain different rates of hydrolysis of (R)- and (S)-quinuclidin-3-yl benzoates and benzoylcholine catalyzed with butyrylcholinesterase, semiempirical PM3 calculations were performed with an assumed active site model of human BChE (20 amino acids). The contributions of different protein residues to the stabilization of Michaelis complexes and tetrahedral intermediates were analyzed. It was shown that the hydrolyses rates of quinuclidinium enantiomers are in an appreciable extent affected by the existence or absence of the hydrogen bond between the quinuclidinium N+-H group and the protein residues. The calculations indicated that the better stabilization of quinuclidinium moiety in the Michaelis complex than in tetraherdal intermediate is the main reason for a greater barrier and a slower reaction rate of the (R)-enantiomer of quinuclidinium esters compared to benzoylcholine. In the case of (S)-enantiomer, the calculation indicated that the barrier for the substrate reorientation from a favourable, but non-productive binding to a productive one, significantly influence the rate of hydrolysis.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA